| Liu Jia,Gao Gang,Wu Shikai,et al.Radiotherapy induced microRNA expression in the plasma of breast cancer patients[J].Chinese Journal of Radiological Medicine and Protection,2016,36(4):260-263,302 |
| Radiotherapy induced microRNA expression in the plasma of breast cancer patients |
| Received:January 06, 2016 |
| DOI：10.3760/cma.j.issn.0254-5098.2016.04.005 |
| KeyWords:Ionizing radiation Breast cancer Radiotherapy miRNA Dose response |
| FundProject: |
| Author Name | Affiliation | E-mail | | Liu Jia | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Gao Gang | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Wu Shikai | 100850 北京, 军事医学科学院附属医院 | | | Piao Chunnan | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Liu Jianxiang | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | jxliu@163.com |
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| Abstract:: |
| Objective To investigate the expressions of miR-210-3p, miR-221-3p, miR-21-5p and miR-150-5p in the plasma of breast cancer patients before and after radiotherapy in order to establish reliable early biomarkers of non-uniform radiation injuries. Methods Blood samples were collected from 13 patients before radiotherapy (0 Gy) and 24 h after radiotherapy of 2, 10, 20, 30 Gy. The miRNAs in the blood plasma were detected with qRT-PCR. Results The levels of miR-210-3p, miR-221-3p, miR-21-5p had no significant difference among different dosage groups after radiotherapy(P>0.05). There was no significant difference between the expression of miR-150-5p before and after 2 Gy radiotherapy(P>0.05). While the relative level of miR-150-5p gradually decreased to 0.808, 0.605, 0.565 (χ2=18.76, P<0.05) with increased accumulative dosage of 10, 20, and 30 Gy, respectively. In addition, the miR-150-5p expression levels had no relationship(P>0.05)with situations (positive or negative group) of human epidermal growth factor receptor 2 (HER2), estrogen receptor (ER) and progesterone receptor (PR) in the breast cancer cells. Conclusions Ionizing radiation could reduce the expression of miR-150 in the plasma of breast cancer patients in a dose-dependent manner. |
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