| Zhang Mengfan,Zhao Hua,Lu Xue,et al.Radiation-induced adaptive response of nucleoplasmic bridges in human peripheral blood lymphocytes[J].Chinese Journal of Radiological Medicine and Protection,2016,36(4):256-259 |
| Radiation-induced adaptive response of nucleoplasmic bridges in human peripheral blood lymphocytes |
| Received:June 07, 2016 |
| DOI：10.3760/cma.j.issn.0254-5098.2016.04.004 |
| KeyWords:Ionizing radiation Nucleoplasmic bridge Adaptive response Priming dose Dose range |
| FundProject:国家自然科学基金(81172593,81573081) |
| Author Name | Affiliation | E-mail | | Zhang Mengfan | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Zhao Hua | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Lu Xue | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Xu Yan | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Feng Jiangbin | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Liu Qingjie | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | qjliu@nirp.cn |
|
| Hits: 3842 |
| Download times: 2758 |
| Abstract:: |
| Objective To explore whether a low dose of 60Co γ-rays could induce the adaptive response in the formation of nucleoplasmic bridges (NPB) in human peripheral blood lymphocytes, and if so, the range of the priming dose. Methods Human peripheral blood samples from healthy males were collected and irradiated with 0, 20, 50, 75, 100, 150 and 200 mGy (dose-rate was 25 mGy/min) of 60Co γ-rays. After 6 h, the samples were irradiated with a challenge dose of 2 Gy (dose-rate was 1 Gy/min). The cytokinesis-block micronucleus(CBMN)assay was carried out to analyze the NPB and micronuclei (MN) formation in binucleated cells. Results Within the dose range of 0-200 mGy,the yields of NPB and MN increased with irradiation dose of γ-rays and the dose response of NPB followed with a linear-quadratic equation of y=(1.5×10-4)x2-(5.67×10-3)x+0.598 (R2=0.893 8). Compared with the samples irradiated with 2 Gy alone, the yields of NPB and MN were significantly reduced when the samples were irradiated with a priming dose of 75-100 mGy before 2 Gy irradiation (U=2.66, 2.97, 3.96, 5.89,P<0.05). The biggest decrease ratio of NPB yields approached to 43.2% at the priming dose of 100 mGy. Conclusions Low doses in the range of 75-100 mGy of 60Co γ-rays could induce the adaptive response of NPB formation in human peripheral blood lymphocytes. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
