| Zhang Qixian,Sun Rui,Xu Meiling,et al.The inhibiting effects of low dose radiation on dendritic growth of newborn neurons in rat hippocampus[J].Chinese Journal of Radiological Medicine and Protection,2016,36(4):252-255 |
| The inhibiting effects of low dose radiation on dendritic growth of newborn neurons in rat hippocampus |
| Received:November 16, 2015 |
| DOI：10.3760/cma.j.issn.0254-5098.2016.04.003 |
| KeyWords:Ionizing radiation Hippocampus New born neurons Dendrite |
| FundProject:国家自然科学基金(81372411,81172128); 江苏省临床医学科技专项(BL2014040) |
| Author Name | Affiliation | E-mail | | Zhang Qixian | Institute of Radiotherapy & Oncology, Soochow University, Suzhou Key Laboratory for Radiation Oncology, Department of Radiotherapy and Oncology, Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | | Sun Rui | Institute of Radiotherapy & Oncology, Soochow University, Suzhou Key Laboratory for Radiation Oncology, Department of Radiotherapy and Oncology, Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | | Xu Meiling | Institute of Radiotherapy & Oncology, Soochow University, Suzhou Key Laboratory for Radiation Oncology, Department of Radiotherapy and Oncology, Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | | Liu Xiaoqian | Institute of Radiotherapy & Oncology, Soochow University, Suzhou Key Laboratory for Radiation Oncology, Department of Radiotherapy and Oncology, Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | | Tian Ye | Institute of Radiotherapy & Oncology, Soochow University, Suzhou Key Laboratory for Radiation Oncology, Department of Radiotherapy and Oncology, Second Affiliated Hospital of Soochow University, Suzhou 215004, China | dryetian@126.com |
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| Abstract:: |
| Objective To investigate the effects of low dose radiation on dendritic growth of newborn neurons in the hippocampus of young rat. Methods One month-old male rats were randomized into radiation group and sham control group. Radiation group received whole brain irradiation at a single dose of 2 Gy. Retrovirus expressing green fluorescent protein (GFP) was used to label newborn neurons in the hippocampus through stereotaxic intracranial infusion. Immunofluorescence assays were performed to detect dendritic architecture alterations induced by irradiation at different time points. Results Compared with control group, the lengths of total dendrite and the longest dendrite significantly decreased at 2 and 4 weeks after irradiation(t=3.10, 2.07, 2.94, 4.02, P<0.05). The branching points of new born neurons were also decreased significantly at 2 weeks post irradiation(t=2.23, P<0.05).The number of new born neurons reduced at 4 weeks post irradiation(t=8.43,P<0.05). Conclusions Low dose radiation could inhibit newborn neuron growth in the hippocampus of young rat, which may be one of the most important mechanisms involved in radiation-induced cognitive impairment. |
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