| Lu Shan,Ke Yuan,Wang You,et al.Radiosensitization of clioquinol and zinc in human cervical cancer cell lines[J].Chinese Journal of Radiological Medicine and Protection,2016,36(4):241-245 |
| Radiosensitization of clioquinol and zinc in human cervical cancer cell lines |
| Received:October 13, 2015 |
| DOI：10.3760/cma.j.issn.0254-5098.2016.04.001 |
| KeyWords:Radiosensitization Clioquinol Zinc HeLa cell NF-κB |
| FundProject:国家自然科学基金(81201755) |
| Author Name | Affiliation | E-mail | | Lu Shan | Department of Chemoradiotherapy of Zhongnan Hospital, Wuhan University, Wuhan 430071, China | | | Ke Yuan | Department of Chemoradiotherapy of Zhongnan Hospital, Wuhan University, Wuhan 430071, China | | | Wang You | Department of Chemoradiotherapy of Zhongnan Hospital, Wuhan University, Wuhan 430071, China | | | Zhao Hong | Department of Chemoradiotherapy of Zhongnan Hospital, Wuhan University, Wuhan 430071, China | | | Gao Xiaojia | Department of Chemoradiotherapy of Zhongnan Hospital, Wuhan University, Wuhan 430071, China | | | Yu Haijun | Department of Chemoradiotherapy of Zhongnan Hospital, Wuhan University, Wuhan 430071, China | | | Zhou Fuxiang | Department of Chemoradiotherapy of Zhongnan Hospital, Wuhan University, Wuhan 430071, China | happyzhoufx@sina.com |
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| Abstract:: |
| Objective To investigate the radiosensitization effects of the combination treatment of clioquinol (CQ) and zinc on human cervical cell line HeLa in vitro. Methods Cells were divided into the 4 groups: controls, drug, radiation, and combined drug and radiation group. Cytotoxic effect of CQ and zinc on cell viability was determined by CCK-8 assay. Radiosensitization effect of CQ and zinc on HeLa cells was detected by colongenic assay, and the single-hit multi-target model was used to stimulate the dose-response curve of survival and to calculate radiosensitization parameters. The cell cycle and apoptosis of HeLa cells were analyzed with flow cytometry. Luciferase reporter assay was used to study NF-κB activity of HeLa cells. Results The combination of CQ and zinc inhibited cell growth in a dose-dependent manner(F=188.00, P<0.01). The mean lethal dose was 3.16 and 2.04 Gy for radiation group and combined drug and radiation group, respectively, and hence the SER was 1.55. Compared with the radiation group, the ratio of G2-phase cells in the combined drug and radiation group decreased(t=10.39, P<0.05), the apoptosis rate increased at 24 h post-irradiation(t=5.64, P<0.01), and the NF-κB activity decreased(t=21.42, P<0.05). Compared to the control group, the NF-κB activity increased in the radiation group(t=6.23, P<0.05), but decreased in the drug group(t=12.48, P<0.05). Conclusions The combination of CQ and zinc could increase the radiosensitivity of HeLa cells by decreasing the ratio of G2-phase cells, increasing apoptosis and the inhibiting of NF-κB activity. |
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