| Li Chengxia,Li Wei,Zhang Fuhai,Jia Qiang,Li Ning,Chang Jin,Ji Yanhui,Tan Jian.Targeting therapeutic effect of radioiodine-labeled anti-EGFR binding nanoparticles on glioblastoma cells[J].Chinese Journal of Radiological Medicine and Protection,2016,36(3):161-167 |
| Targeting therapeutic effect of radioiodine-labeled anti-EGFR binding nanoparticles on glioblastoma cells |
| Received:August 03, 2015 |
| DOI：10.3760/cma.j.issn.0254-5098.2016.03.001 |
| KeyWords:Nanoparticles Radioiodine-labeled cells Epidermal growth factor receptor antibody 131I |
| FundProject:国家自然科学基金(81301244) |
| Author Name | Affiliation | E-mail | | Li Chengxia | Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China | | | Li Wei | Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China | | | Zhang Fuhai | Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China | | | Jia Qiang | Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China | | | Li Ning | Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China | | | Chang Jin | Institute of Nanobiotechnology, School of Materials Science and Engineering, Tianjin Key Laboratory of Composites and Functional Materials, Tianjin University, Tianjin 300072, China | | | Ji Yanhui | Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China | | | Tan Jian | Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China | tanpost@163.com |
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| Abstract:: |
| Objective To explore the feasibility of treating glioma with 131I-labeled antiEGFR nanoparticales in vitro and in vivo. Methods Radioiodine-labeled anti-EGFR binding nanoparticles were constructed and its in vitro cell-binding ability was confirmed by confocal microscopy and flow cytometry. Cell cytotoxicity of the drug was evaluated by MTT assay. Radioiodine-imaging studies were conducted by using a xenograft nude mouse model in vivo by detecting the change of the volume of the xenograft. ResultsIn vitro studies revealed that the anti-EGFR nanoparticles binding with bovine serum albumin-polycaprolactone (anti EGFR-BSA-PCL) or BSA-PCL were successfully constructed. Cells could uptake 131I-antiEGFR-BSA-PCL much more effectively than the 131I-BSA-PCL group. MTT assay indicated that, when the radioactivity approached to 0.925 MBq, the cell growth inhibition rate of 131I-antiEGFR-BSA-PCL was higher than that of 131I-BSA-PCL (U251 cells: t=2.517, P<0.05; U87 cells: t=2.821, P<0.05). The growth inhibition rates at 0.925 MBq was higher than other radioactivity in both U251 cells and U87 cells (131I-antiEGFR-BSA-PCL group: t=2.148, 2.436, P<0.05; 131I-BSA-PCL group: t=2.693, 2.615, P<0.05). Radioactive iodine uptake assay showed that the viability of U251 and U87 cells was reduced after exposure to 0.37 MBq to 3.7 MBq of 131I-antiEGFR-BSA-PCL or 131I-BSA-PCL. The in vivo nude mice experiments disclosed that both kinds of nanoparticles passed by the hepatic metabolism, and the decrease of tumor volume in the group of 131I-antiEGFR-BSA-PCL was more effective than that in the 131I-BSA-PCL group (t=4.115, P<0.05). EGFR significantly enhanced the uptake and accumulation of BSA-PCL in the xenografts nude mice model, indicating an improved nanoparticle-based drug delivery. Conclusions 131I-antiEGFR-BSA-PCL based radioiodine therapy of U251 and U87 cells had good curative effect in vitro and in vivo. Thus, 131I-antiEGFR-BSA-PCL may provide a new method for glioblastoma treatment. |
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