| Ji Yanhui,Li Wei,Li Chengxia,Li Ning,Chang Jin,Tan Jian.Evaluation of the internal therapeutic effectiveness of 131I-antiEGFR-BSA-PCL in nude mice with colorectal cancer[J].Chinese Journal of Radiological Medicine and Protection,2016,36(2):81-86 |
| Evaluation of the internal therapeutic effectiveness of 131I-antiEGFR-BSA-PCL in nude mice with colorectal cancer |
| Received:June 26, 2015 |
| DOI：10.3760/cma.j.issn.0254-5098.2016.02.001 |
| KeyWords:131I Colorectal cancer Nano-liposome Epidermal growth factor receptor |
| FundProject:国家自然科学基金(81301244,81171372) |
| Author Name | Affiliation | E-mail | | Ji Yanhui | Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China | | | Li Wei | Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China | | | Li Chengxia | Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China | | | Li Ning | Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China | | | Chang Jin | Tianjin University, Tianjin 300072, China | | | Tan Jian | Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China | tanpost@163.com |
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| Abstract:: |
| Objective To investigate the biological effects of internal radiation and therapeutic effectiveness of 131I-labeled anti-epidermal growth factor receptor (EGFR) in colorectal cancer of model mice. Methods Nano-liposome characterized for EGFR-targeting was constructed. The efficacy of cellular binding and uptake of the liposome was evaluated by the analysis of confocal microscopy observation and the iodide uptake assay. After intra-tumor injections of 74 MBq (740 MBq/ml) 131I-antiEGFR-BSA-PCL, 131I-BSA-PCL, 131I or an equivalent volume of normal saline. The biological effects of internal irradiation and therapeutic efficacy of the liposomes on colorectal cancer modeled in a male BALB/c mouse were evaluated by means of tumor size, body weight, histopathology, and SPECT imaging. Results The confocal fluorescence images showed that the antiEGFR-BSA-PCL was successfully internalized into LS180 cells. The 131I uptake efficacy of 131I-antiEGFR-BSA-PCL was significantly higher than that of 131I-BSA-PCL in LS180 cells (t=2.77-5.40,P<0.01). Tumor size measurement showed that tumor growth was inhibited by the treatment with 131I-EGFR-BSA-PCL and 131I-BSA-PCL, but had no significant differences between these two groups (P>0.05). It was found that the 131I-antiEGFR-BSA-PCL was markedly taken up by the tumor and reached its uptake value of (21.61±1.01) and (20.58±0.65)%ID/g at 72 h following drug injection, which was higher than the uptake value of 131I (t=9.36, 8.69, P<0.01). SPECT imaging assay showed that, after being injected into mouse tumor, the 131I-EGFR-BSA-PCL and 131I-BSA-PCL were uniformly distributed inside the tumor. Conclusions 131I-antiEGFR-BSA-PCL obviously suppresses the development of colorectal cancer in mice. |
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