| Hu Chao,Qian Danqi,Qin Songbing,et al.Radiosensitization effect of low-temperature plasma on human malignant cells[J].Chinese Journal of Radiological Medicine and Protection,2015,35(11):819-824 |
| Radiosensitization effect of low-temperature plasma on human malignant cells |
| Received:December 31, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.11.005 |
| KeyWords:Radiosensitization Low-temperature plasma HepG2 cells HeLa cells A549 cells |
| FundProject: |
|
| Hits: 3011 |
| Download times: 2380 |
| Abstract:: |
| Objective To evaluate the radiosensitization effect of low-temperature plasma on HepG2, A549, and HeLa cells.Methods Cells were divided into three groups, radiation group(R), plasma treatment group(P), and plasma plus radiation group(P+R). After radiation, cell survival was detected by a cloning assay. Cell cycle distribution, apoptosis and ROS content were tested by flow cytometry. Western blot was used to measure the expressions of Caspase-3 and Bcl-2. Results Low-temperature plasma showed radiosensitization effects on three different human malignant cell lines with a sensitivity enhancement ratio(SERD0)of 1.28,1.32 and 1.29. respectively. In these three different human malignant cell lines, compared with radiation alone group(R), the G2/M arrest, apoptosis rate and ROS level in the group P+R were enhanced(the prolongation of G2/M arrest:t=9.52, 8.24, 9.53, P<0.05;the apoptosis rate:t=10.67, 38.56, 6.74,P<0.05;ROS content:t=9.41, 15.42, 13.53, P<0.05). In HepG2 cells and A549 cells, compared with group P, the prolongation of G2/M arrest, the apoptosis rate and ROS content of group P+R were enhanced(the prolongation of G2/M arrest:t=8.75, 20.37, P<0.05;the apoptosis rate:t=8.43, 9.99, P<0.05;ROS content:t=4.82, 5.27, P<0.05). The expression level of Bcl-2 protein was downregulated in group P+R;by contrast, the expression level of Caspase-3 protein in group P+R was upregulated. Conclusions Low-temperature plasma can increase the radiosensitization of HepG2, A549 and HeLa cells with the enhancement of G2/M phase arrest, apoptosis induction and ROS generation. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
