| Xie Kaihong,Hu Xiaomao,Liu Pingping,et al.Role of miR-155 in radioresistance of lymphoma Raji cells[J].Chinese Journal of Radiological Medicine and Protection,2015,35(11):801-804 |
| Role of miR-155 in radioresistance of lymphoma Raji cells |
| Received:May 23, 2015 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.11.001 |
| KeyWords:miR-155 Burkitt lymphoma Radiation |
| FundProject:国家自然基金(81270568) |
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| Abstract:: |
| Objective To investigate the role of miR-155 in radio-resistance of lymphoma cell line Raji cells.Methods The expression of miR-155 was detected by quantitative real-time PCR, cell growth was detected by CCK-8 assay, cell apoptosis was detected by flow cytometry, and the protein expressions of PTEN and p-AKT were detected by Western blot. Results The Raji cells had high levels of miR-155 RNA and p-AKT protein had a very low level of PTEN protein. Cell apoptosis was induced by 4.0 Gy X-ray irradiation after 48 h. The transfection of cells with miR-155 siRNA not only silenced miR-155, but also significantly decreased p-AKT level, increased the expression of pten gene and protein level, inhibited cell proliferation, and increased the radiation sensitivity of Raji cell, the apoptosis rate of siRNA+ irradiation group(36.78%±1.35%) was higher than that of irradiation group(t=12.572,P<0.05).Conclusions High levels of miR-155 was involved in radiation resistance of lymphoma Raji cells and this activity is likely modulated by the PTEN/PI3K/AKT signaling pathway. |
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