| Liu Dongmei,Wu Hui,Ma Yanhui,Zhang Wenguang.Effects of up and down-regulation of Cox-2 expression on radiation sensitivity of human esophageal cancer EC9706 xenograft in nude mice[J].Chinese Journal of Radiological Medicine and Protection,2015,35(10):734-737 |
| Effects of up and down-regulation of Cox-2 expression on radiation sensitivity of human esophageal cancer EC9706 xenograft in nude mice |
| Received:June 17, 2015 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.10.004 |
| KeyWords:Esophageal cancer EC9706 Cox-2 Xenograft tumor Nude mice |
| FundProject:河南省医学科技攻关计划项目(200903144) |
|
| Hits: 2662 |
| Download times: 2122 |
| Abstract:: |
| Objective To explore the effects of up- and down-regulation of Cox-2 expression on the growth and radiation sensitivity of human esophageal cancer EC9706 xenograft in nude mice. Methods Cox-2 specific siRNA and Cox-2 gene eukaryotic expression vector were constructed and transfected to esophageal cancer cells EC9706, and the stable transfected cell lines were obtained by the method of G418 screening. The expressions of Cox-2 mRNA and its protein were detected by RT-PCR and Western blot, respectively. The inhibitory effects of Cox-2 regulation combined with X-ray irradiation on cancer cell growth were detected by the nude mouse xenograft assay. Results Cox-2 gene expression was significantly decreased in the Cox-2 down-regulated group and increased in the Cox-2 up-regulated group. Compared with the control group without gene transfer, the average volumes of EC9706 xenograft tumor in the Cox-2 up- and down-regulated group significantly decreased (F=34.26,P<0.05) and increased (F=26.38,P<0.05), respectively. After 20 Gy X-ray irradiation, the average volume of xenograft was significantly reduced in the Cox-2 down-regulated group (F=16.35,P<0.05), but it had no significantly changes in the Cox-2 up-regulated group. Conclusions Down-regulation of Cox-2 expression inhibits the growth of human esophageal cancer EC9706 xenograft in a nude mice and increases cell radiation sensitivity, but up-regulation of Cox-2 expression makes tumor cells to become radioresistant. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
