| Luo Xiaoxu,Xiao Lei,Ainiwaer·Aimudula,Wu Ge,Wang Yunlian,Zhang Hua,Bao Yongxing.Relationship of PI3K/Akt signaling pathway and the activation of hepatic stellate cells and its role in radiation-induced hepatic fibrosis[J].Chinese Journal of Radiological Medicine and Protection,2015,35(9):652-656 |
| Relationship of PI3K/Akt signaling pathway and the activation of hepatic stellate cells and its role in radiation-induced hepatic fibrosis |
| Received:March 25, 2015 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.09.003 |
| KeyWords:X-rays Hepatic stellate cells Radiation-induced hepatic fibrosis PI3K/Akt signaling pathway |
| FundProject:新疆维吾尔自治区自然科学基金(2014211C075) |
| Author Name | Affiliation | E-mail | | Luo Xiaoxu | Department of Oncology, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China | | | Xiao Lei | Department of Oncology, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China | | | Ainiwaer·Aimudula | Department of Oncology, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China | | | Wu Ge | Department of Oncology, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China | | | Wang Yunlian | Department of Oncology, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China | | | Zhang Hua | Department of Oncology, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China | | | Bao Yongxing | Department of Oncology, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China | baoyx@vip.sina.com |
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| Abstract:: |
| Objective To investigate the relationship of PI3K/Akt signaling pathway with the activation of hepatic stellate cells (HSC) and its role in radiation-induced hepatic fibrosis. Methods HSC was treated with 6 MV X-ray irradiation (IR) together with the inhibitor of PI3K/Akt signaling pathway. The cells were divided into inhibitor group, 10 Gy IR group, 10 Gy+inhibitor group, 20 Gy IR group, an 20 Gy+inhibitor group and blank control group. Then cell apoptosis rate was detected, the expression of transforming growth factor β1 (TGF-β1) in cell supernatant and the mRNA expressions of α-smooth muscle actin (α-SMA) and phosphorylation protein kinase B (p-Akt) were measured. Results Compared with the control group, the apoptosis rate of 10 and 20 Gy IR group increased with irradiation dose (t=8.43, 11.63, P<0.05) but they were reduced by the inhibitor of PI3K/Akt (t=8.09, 4.88, P<0.05). The expressions of TGF-β1, α-SMA, and p-Akt also increased with irradiation dose (t=6.91, 7.80, 9.28, P<0.05) but they were declined by this inhibitor for both 10 Gy IR (t=6.17, 15.11, 10.34, P<0.05) and 20 Gy IR (t=10.04, 6.85, 23.84, P<0.05). Conclusions X-ray irradiation could activate HSC through PI3K/Akt signaling pathway, which may further result in hepatic fibrosis. |
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