| Liu Hong,Wang Yujing,Bian Lei,et al.Resistance of CD44+/CD24+ expressing cervical cancer cells on apoptosis induced by X-ray irradiation[J].Chinese Journal of Radiological Medicine and Protection,2015,35(9):647-651 |
| Resistance of CD44+/CD24+ expressing cervical cancer cells on apoptosis induced by X-ray irradiation |
| Received:April 01, 2015 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.09.002 |
| KeyWords:Cervical cancer Cancer stem cells Apoptosis Radioresistance |
| FundProject:河北省科技支撑计划项目(14277770D);河北省杰出青年科学基金(C2010002011) |
| Author Name | Affiliation | E-mail | | Liu Hong | Department of Gynecological Tumor, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China | | | Wang Yujing | Department of Gynecological Tumor, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China | | | Bian Lei | Department of Gynecological Tumor, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China | | | Zhang Qianying | Department of Gynecological Tumor, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China | | | Fang Zhaohui | Department of Gynecological Tumor, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China | | | Wu Xiaohua | 石家庄国际和平医院妇产科 | | | Cheng Jianxin | Department of Gynecological Tumor, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China | jianxin_cheng@263.net |
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| Abstract:: |
| Objective To explore whether CD44+/CD24+ expressing cervical cancer cells are resistant to X-ray irradiation and investigate the underlying mechanism. Methods Cervical cancer cell line (Siha) was cultured in vitro and the CD44+/CD24+ expressing cells were sorted with a flow cytometer. The cells were irradiated with 8, 16 and 30 Gy of 6 MV X-rays. Colony formation test was used to evaluate the radiosensitivity of CD44+/CD24+ expressing cervical cancer cells. Cell morphology was observed by electronmicroscopy, cell apoptosis was analyzed with a flow cytometer and also verified with a DNA "ladder" assay. Gene expression was determined by RT-PCR. Results After radiation, the ratio of CD44+/CD24+ cells significantly increased. Compared to Siha cells, the radiosensitivity of CD44+/CD24+ cells decreased (t=93.99-400.45, P<0.05), and the expressions of bcl-2, survivin and Oct4 mRNA increased in CD44+/CD24+ cells (t=221.35, 941.65, 82.27, P<0.01). Both apoptotic body and specific DNA "ladder" pattern were observed in cells but not in the CD44+/CD24+ Sihacells which had no obvious morphological changes of apoptosis. Conclusions The CD44+/CD24+ expressing cervical cancer cells are resistant to X-rays due to expression of anti-apoptosis factors. |
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