| Zhao Zhenzhen,Mao Aiwu,Wang Zhongmin,et al.The biological effects of 125I seeds and 60Co γ-rays on A549 and BEAS-2B cells[J].Chinese Journal of Radiological Medicine and Protection,2015,35(8):575-579 |
| The biological effects of 125I seeds and 60Co γ-rays on A549 and BEAS-2B cells |
| Received:December 30, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.08.004 |
| KeyWords:125I seeds Low dose rate 60Co High dose rate Non-small cell lung cancer Cell apoptosis |
| FundProject:上海市卫计委科研项目(20114014);中国博士后科学基金(2015T80489) |
| Author Name | Affiliation | E-mail | | Zhao Zhenzhen | Department of Radiology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200001, China | | | Mao Aiwu | 上海交通大学医学院附属同仁医院介入科 | | | Wang Zhongmin | 东南大学医学院, 上海交通大学医学院附属瑞金医院卢湾分院 | wzm0722@hotmail.com | | Liu Fenju | 苏州大学医学部放射医学与防护学院 | | | Cao Yan | Department of Radiology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200001, China | | | Jia Yiping | Department of Radiology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200001, China | |
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| Abstract:: |
| Objective To investigate the biological effects of 125I seeds and 60Co γ-rays on the non-small cell lung cancer cells A549 and the normal bronchial epithelium cells BEAS-2B. Methods A549 and BEAS-2B cells were irradiated with 125I seeds and 60Co γ-rays. The survival fraction was detected by colony formation assay. The cell cycle and cell apoptotic ratio were detected by flow cytometry. The expression of cell apoptotic related proteins was examined by western blot. Results After irradiation with different doses, the survival of A549 cells irradiated with 125I seeds was lower than that irradiated with γ-rays (t=6.06, 9.42, 4.90,P<0.05). After irradiation with 4 Gy of 125I seeds and 60Co γ-rays, the G1 phase percentages of A549 cells were 70.67%±1.49% and 59.59%±0.71% (t=10.77,P<0.05), and the apoptotic ratios of A549 cells were 18.09%±0.73% and 9.81%±0.16% (t=19.40,P<0.05), respectively. 125I seeds irradiation remarkably up-regulated the expressions of Bax and cleaved Caspase-3 proteins, down-regulated the expression of Bcl-2 proteins compared with 60Co γ-rays irradiation on A549 cells. However, the apoptotic ratio and the expressions of apoptosis-related proteins in BEAS-2B cells had little difference between two types of radiation. Conclusions The anti-proliferative effect of 125I seeds irradiation on A549 cell is more remarkably than that of 60Co γ-rays. The imbalance of Bcl-2/Bax ratio and the eventually activation of Caspase-3 proteins may play an important role in the anti-proliferative effect induced by the continuous low dose radiation of 125I-seeds. |
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