| He Ru,Li Xiaoyang,Sun Rui,Tian Ye.Effect of X-ray irradiation on the neurites growth of primary hippocampal neurons[J].Chinese Journal of Radiological Medicine and Protection,2015,35(8):570-574 |
| Effect of X-ray irradiation on the neurites growth of primary hippocampal neurons |
| Received:March 06, 2015 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.08.003 |
| KeyWords:X-rays Neuron Neurite Hippocampus In vitro |
| FundProject:国家自然科学基金(81372411,81172128);江苏省临床医学科技专项资助项目(BL2014040);江苏省研究生创新工程项目(CXZZ13_0838) |
| Author Name | Affiliation | E-mail | | He Ru | Department of Radiation Oncology, Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | | Li Xiaoyang | Department of Radiation Oncology, Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | | Sun Rui | Department of Radiation Oncology, Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | | Tian Ye | Department of Radiation Oncology, Second Affiliated Hospital of Soochow University, Suzhou 215004, China | dryetian@126.com |
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| Abstract:: |
| Objective To investigate the effect of X-ray irradiation on neurites growth of primary hippocampal neurons in vitro. Methods Primary hippocampal neuron culture in vitro were irradiated with 0, 2, 4, 8, 10, 12 Gy of X-rays. In the first day and the third day after irradiation, the cell death of primary hippocampal neurons was detected by MTT method, and the morphological changes of primary hippocampal neuronal neurites were detected with immunofluorescence staining method. Results In the first day and third day after irradiation, the cell death of hippocampal neurons increased significantly (F=123.068, 43.370,P<0.05), but there were no significant difference among 4, 8, 10, and 12 Gy irradiation groups. Immunofluorescence staining showed that, in the first day after irradiation, the neurite length and total dendritic branch length (TDBL) were significantly changed (F=9.169, 7.856, P<0.05), and in the third day after radiation,the neurite length, TDBL and total dendritic branch tip number (TDBTN) were also altered (F=23.797, 6.565, 6.021, P<0.05). Conclusion X-ray irradiation can inhibit the growth of neurites in the primary hippocampal neurons in vitro. |
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