| Yue Haiying,Hu Kai,Jiang Jian,Chen Yuhua,Liu Wenqi,Wang Rensheng.The study on lung injury of minipigs' right-thorax with a single high-dose γ-ray irradiation[J].Chinese Journal of Radiological Medicine and Protection,2015,35(8):561-565 |
| The study on lung injury of minipigs' right-thorax with a single high-dose γ-ray irradiation |
| Received:January 06, 2015 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.08.001 |
| KeyWords:Radiotherapy Minipigs Alveolar epithelial type Ⅱ cells Epithelial-mesenchymal transition |
| FundProject:人口健康与食品安全关键技术研究开发基金(桂科攻1140003A-24) |
| Author Name | Affiliation | E-mail | | Yue Haiying | Department of Radiotherapy, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China | | | Hu Kai | Department of Radiotherapy, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China | | | Jiang Jian | Department of Radiotherapy, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China | | | Chen Yuhua | 广西壮族自治区妇幼保健院病理科 | | | Liu Wenqi | Department of Radiotherapy, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China | | | Wang Rensheng | Department of Radiotherapy, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China | 13867806008@163.com |
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| Abstract:: |
| Objective To explore the influence and mechanism of cytokines and protein expression of alveolar epithelial type (ACE) II cells in Bama minipigs' right-thorax with a single 15 Gy dose irradiation. Methods All minipigs received either right thoracic irradiation or sham-irradiation under anesthesia. At 4, 8, 12 and 24 week post-irradiation, 5 minipigs respective and random from irradiarion groups and control group were sacrificed to remove the lungs. The protein expression of surfactant associated protein (SP)-A, transforming growth factor (TGF)-β1, Vimentin and E-cadherin were detected by Western blot. The protein expression of α-smooth muscle actin (SMA) was detected by immunohistochemistry. The co-localization of SP-A and α-SMA was visualized by double immunofluorescence staining. Results At 4, 8, 12 and 24 week post-irradiation, a significant increase in the protein expression of α-SMA, TGF-β1 and Vimentin were observed in irradiated lung compared to sham-irradiated controls(α-SMA: t=2.46-3.26, P<0.05; TGF-β1: t=2.96-3.52, P<0.05; Vimentin: t=3.24-5.05, P<0.05). By contrast, the protein expression of SP-A and E-cadherin in irradiation group was lower than it in control group(SP-A: t=3.62-4.65,P<0.05;E-cadherin:t=2.53-4.15,P<0.05). Moreover, at 8 week after irradiation, under confocal laser scanning microscope, the co-localization of SP-A and α-SMA was observed in irradiated alveolar epithelium cells, and it was not observed in sham-irradiated controls. Conclusions These data demonstrate that E-cadherin, SP-A and TGF-β1 may act as sensitive predictors of radiation-induced lung injury(RILI). Irradiation may lead to ACEⅡ cells achieving a mesenchymal phenotype, namely, epithelial to mesenchymal cells transition occurs, and ACEⅡ cells play the important part in the development of RILI by epithelial-mesenchymal transition. |
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