| Wang Junke,Han Aruna,Wang Limei,Yu Zuyin,Xu Tiezhu,Yang Chao,Shan Yajun,Cui Yu,Liu Xiaolan,Cong Yuwen.The influence of a single high dose of rhG-CSF on hematopoietic reconstitution of mice with nonmyeloablative bone marrow transplantation[J].Chinese Journal of Radiological Medicine and Protection,2015,35(7):501-504 |
| The influence of a single high dose of rhG-CSF on hematopoietic reconstitution of mice with nonmyeloablative bone marrow transplantation |
| Received:February 05, 2015 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.07.005 |
| KeyWords:Nonmyeloablative Bone marrow transplantation Hemopoietic stell cell rhG-CSF |
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| Abstract:: |
| Objective To observe the peripheral hematopoietic recovery after a high dose of rhG-CSF administration on the mice with nonmyeloablative bonw marrow transplantation (BMT), and provide new experimental evidence for the clinical application of rhG-CSF. Methods Male C57 mice were treated with rhG-CSF at 0.5 h after whole body γ-ray irradiation and then were randomly divided into 5 groups with different time of BMT after irradiation (vehicle, 0.5, 6, 24, 48 h) and 4 groups with different dosage of rhG-CSF (0, 0.25, 0.5, and 1 mg/kg). The amount of karyocyte was analyzed to evaluate the best BMT time and dosage of rhG-CSF. To explore the effect of rhG-CSF on the implantation of exogenous stem/progenitor cells, a model of BMT with GFP+ fluorescent mice was established, in which the percentage of GFP+ leucocyte cells of the total peripheral blood nucleated cells of recipient mice was tested by flow cytometry. Results The administration of rhG-CSF could accelerate multi-lineage hematopoietic recovery, including white blood cells, red blood cells and platelets on mice with nonmyeloablative BMT (F=17.76,14.75,8.66, P<0.05). The optizum time of BMT was 24 h after irradiation and the best dosage of rhG-CSF was 1 mg/kg (F=5.34,8.92,16.54,P<0.05). In addition, in the irradiated mice receiving BMT with GFP mouse cells, the amount of peripheral white blood cells in the rhG-CSF group was significantly higher than those of control mice in 9~21 d (F=35.61, P<0.05), while the proportion of GFP+ cells between these two groups had no significant difference. Conclusions A single high dose of rhG-CSF can significantly promote multi-lineage hematopoietic recovery on mice with nonmyeloablative BMT, while the implantation rate of exogenous hematopoietic stem cells has no obvious influence. |
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