| Lu Xiaoxu,Wu Hui,Xu Jing,et al.Preliminary study on the correlation between regulation of Cox-2 gene expression and radiosensitivity of esophageal cancer[J].Chinese Journal of Radiological Medicine and Protection,2015,35(7):496-500 |
| Preliminary study on the correlation between regulation of Cox-2 gene expression and radiosensitivity of esophageal cancer |
| Received:September 29, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.07.004 |
| KeyWords:Cox-2 gene Esophageal cancer Radiosensitivity Mechanism |
| FundProject:河南省科技攻关计划 |
| Author Name | Affiliation | E-mail | | Lu Xiaoxu | Department of Radiation, Affiliated Tumor Hospital of Zhengzhou University, Henan Tumor Hospital, Zhengzhou 450008, China | | | Wu Hui | Department of Radiation, Affiliated Tumor Hospital of Zhengzhou University, Henan Tumor Hospital, Zhengzhou 450008, China | wuhui7008@126.com | | Xu Jing | Department of Radiation, Affiliated Tumor Hospital of Zhengzhou University, Henan Tumor Hospital, Zhengzhou 450008, China | | | Wang Yanling | Department of Radiation, Affiliated Tumor Hospital of Zhengzhou University, Henan Tumor Hospital, Zhengzhou 450008, China | | | Huang Rong | Department of Radiation, Affiliated Tumor Hospital of Zhengzhou University, Henan Tumor Hospital, Zhengzhou 450008, China | |
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| Abstract:: |
| Objective To investigate the mechanism of the radiosensitivity effect of Cox-2 gene in esophageal cancer. Methods Cox-2 specific siRNA was constructed and transfected to EC9706 cells to downregulate intracellular Cox-2 expression. The expressions of MMP-2, Bcl-2 mRNA, AKT and phosphorylated AKT proteins were assayed after radiation. Colony formation, cell proliferation, apoptosis and cell invasion in vitro were examined as well. One-way ANOVA method was used to analyze the data. Results After 2 and 4 Gy irradiation, a significant increase in the mRNA expression of Bcl-2 was observed in the Cox-2 up-regulation group(F=3.36,4.32,P<0.05). In the group of Cox-2 downregulation, the expression of MMP-2 mRNA was significantly reduced(F=3.86,8.09,P<0.05). After irradiation, a significant decrease of Bcl-2 mRNA(F=3.73,5.64,P<0.05) as well as an increase of Bax(F=7.03,7.42,P<0.05)was detected, and the levels of total and phosphorylated AKT proteins had the highest level in the Cox-2 upregulation group and had the lowest level in the Cox-2 downregulation group. In the Cox-2 downregulation group, the apoptosis induction obviously increased with dose(F=317.40,P<0.05), and the proportion of cells in G0-G1 phase gradually increased but the proportion of cells in S and G2-M phases decreased, concomitant with the obvious suppression of cell proliferation, in addition, cell invasion was decreased. Conclusions Downregulation of intracellular Cox-2 mRNA expression, concomitant with subsequent downregulation of MMP-2 and Bcl-2 and upregulation of Bax, resulted in reduction of the invasion and metastatic capabilities of tumor cells, and induction of G0-G1 phase arrest and apoptosis. Downregulation of AKT and phosphorylated AKT (pAKT) protein expression might also interfere with the capability of the PI3K/Akt signal transduction pathway to resist radiotherapy. |
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