| Lu Xiaodi,Zhu Xiaodong,Zhao Wei,Qu Song,Liang Xia,Su Fang.Suppression of autophagy activity enhances the radiosensitivity of human nasopharyngeal carcinoma CNE-2 cells[J].Chinese Journal of Radiological Medicine and Protection,2015,35(6):423-427 |
| Suppression of autophagy activity enhances the radiosensitivity of human nasopharyngeal carcinoma CNE-2 cells |
| Received:November 17, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.06.005 |
| KeyWords:ATG5 Autophagy Nasopharyngeal carcinoma Radiosensitivity |
| FundProject:国家自然科学基金(81160285);广西壮族自治区自然科学基金(桂科自2010gxnsfa013240) |
| Author Name | Affiliation | E-mail | | Lu Xiaodi | Department of Radiotherapy, The Affiliated Tumor Hospital of Guangxi Medical University, 530021 Nanning, China | | | Zhu Xiaodong | Department of Radiotherapy, The Affiliated Tumor Hospital of Guangxi Medical University, 530021 Nanning, China | zhuxiaodong83@163.com | | Zhao Wei | Department of Radiotherapy, The Affiliated Tumor Hospital of Guangxi Medical University, 530021 Nanning, China | | | Qu Song | Department of Radiotherapy, The Affiliated Tumor Hospital of Guangxi Medical University, 530021 Nanning, China | | | Liang Xia | Department of Radiotherapy, The Affiliated Tumor Hospital of Guangxi Medical University, 530021 Nanning, China | | | Su Fang | Department of Radiotherapy, The Affiliated Tumor Hospital of Guangxi Medical University, 530021 Nanning, China | |
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| Abstract:: |
| Objective To investigate the effect of autophagy in radiosensitivity of nasopharyngeal carcinoma CNE-2 cells. Methods The expression of ATG5 in CNE-2 cells was inhibited by lentiviral mediated RNA interference. The cells were divided into three groups: control group, NC group and ATG5 group. Cell proliferation, apoptosis and radiosensitivity were detected by CCK-8 method, flow cytometry and colony-forming assay, respectively. Results Compared with the NC group and control group, the survival of ATG5-interfected cells was reduced (F=3.755,46.086,8.609,44.160,P<0.05). After 6 Gy X-ray irradiation, the apoptosis index of the ATG5 group significantly higher than that of NC group and control group (F=394.876,P<0.05). In addition, the radiosensitivity of ATG5 group was also higher than that of control cells. Conclusions Suppression of autophagy activity enhances the radiosensitivity of human nasopharyngeal carcinoma cells. |
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