| Zhang Jian,Zhang Ruiguang,Cai Qian,Chen Lingjuan,Dong Jihua,Wu Gang,Dong Xiaorong.Shenqi Fuzheng Injection (SFI) inhibits irradiation-induced brain injury[J].Chinese Journal of Radiological Medicine and Protection,2015,35(6):419-422 |
| Shenqi Fuzheng Injection (SFI) inhibits irradiation-induced brain injury |
| Received:October 16, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.06.004 |
| KeyWords:Shengqi Fuzheng injection Irradiation induced acute brain injury Inflammatory factors Microglia activation NF-κB |
| FundProject:国家自然科学基金(30800283,81172595);中国博士后科学基金(20100480905);中国临床肿瘤学科学基金(Y-L2011-002) |
| Author Name | Affiliation | E-mail | | Zhang Jian | Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430023, China | | | Zhang Ruiguang | Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430023, China | | | Cai Qian | Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430023, China | | | Chen Lingjuan | Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430023, China | | | Dong Jihua | 430023 武汉, 华中科技大学同济医学院附属协和医院中心实验室 | | | Wu Gang | Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430023, China | | | Dong Xiaorong | Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430023, China | xr.dong@gmail.com |
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| Abstract:: |
| Objective To explore the effect of SFI in radiation-induced mice brain injury after 20 Gy cranial radiation. Methods The mice were divided into three groups: (1) control group,(2) RT-only group:the whole brain was irradiated with a dose of 20 Gy, (3) RT and SFI group: SFI at 20 ml/kg/d from 4 weeks after 20 Gy cranial radiation theraty(CRT). Results Morris water maze test showed that the latency of the irradiated group was longer than control group and SFI improved the cognitive function of mice(t=6.34,6.70,P<0.05). The expression of TNF-α reached to the highest level at 3 h after irradiation, and then it decreased but got the second higher level again at 4 weeks after irradiation. The expression of IL-1β reached to the highest level at 72 h after irradiation and decreased until 4 weeks after irradiation. SFI decreased both expressions of TNF-α(t=11.34,9.70,6.07,P<0.05)and IL-1β(t=12.27,5.70,7.52,P<0.05). Immune florescence staining showed that SFI reduced the number of activated microglia(t=12.35,8.64,7.82,P<0.05)and inhibited the translocation of p65 of microglia after irradiation. Conclusions Findings suggest that SFI may decrease microglial activation and suppress the expression of TNF-α and IL-1β by inhibiting the translocation of NF-κB p65 and then attenuate irradiation-induced brain injury. |
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