| Guo Ya,Zhu Xiaodong,Qu Song,Li Ling,Ge Lianying,Huang Shiting,Zhao Wei,Su Fang,Li Ye.Silencing STAT1 gene enhances radiosensitivity of radioresistant human nasopharyngeal carcinoma CNE-2R cells[J].Chinese Journal of Radiological Medicine and Protection,2015,35(5):334-338 |
| Silencing STAT1 gene enhances radiosensitivity of radioresistant human nasopharyngeal carcinoma CNE-2R cells |
| Received:November 08, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.05.004 |
| KeyWords:STAT1 RNA interference Lentiviral vector Radiosensitivity Nasopharyngeal carcinoma |
| FundProject:国家自然科学基金(30860329);广西壮族自治区自然科学基金(桂科自0832229);广西重点实验室专项经费资助项目(GK2014-ZZ09);广西高等学校重大科研资助项目(201101ZD004) |
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| Abstract:: |
| Objective To study the effect of silencing STAT1 on radiation sensitivity of nasopharyngeal carcinoma cells. Methods Lentivirus-mediate STAT1 gene-transfer was performed to radioresistant human nasopharyngeal carcinoma CNE-2R cells and the transfection efficiency was detected by RT-PCR. The cell proliferation was measured by MTT, and the apoptosis was analyzed by flow cytometry. The radiosensitization ratio was measured according to the clone formation efficiency. Results After transfection with STAT1 siRNA, the expression of STAT1 was effectively inhibited in CNE-2R cells(F=429.87, P<0.05), the cell proliferation was decreased (F=3.88-4.63, P<0.05), the apoptosis was increased (F=38.13,P<0.05), the cell radiosensitivity was enhanced (F=252.80,P<0.05), but the cell cycle distribution at G0/G1, S and G2/M were not influenced (P>0.05).Conclusions Silencing STAT1 could increase radiosensitization of nasopharyngeal carcinoma cells. |
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