| Wang Jing,Zhang Yaping,Ding Defang,et al.Evaluation of applying γ-H2AX as a radiation biodosimetry with an animal model[J].Chinese Journal of Radiological Medicine and Protection,2015,35(5):329-333 |
| Evaluation of applying γ-H2AX as a radiation biodosimetry with an animal model |
| Received:January 23, 2015 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.05.003 |
| KeyWords:γ-rays lymphocytes γ-H2AX pATM(S1981) pDNA-PKcs(T2609) |
| FundProject:国家自然科学基金(81273000) |
| Author Name | Affiliation | E-mail | | Wang Jing | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | Zhang Yaping | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | Ding Defang | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | Gao Yun | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | Zhang Xuxia | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | Zhang Junxiang | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | Chen Honghong | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | hhchen@shmu.edu.cn |
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| Abstract:: |
| Objective To evaluate the potential feasibility of γ-H2AX foci as a biodosimetry after exposure to ionizing radiation by comparing DNA double-strand break repair kinetics in rat blood lymphocytes with that in human lymphocytes. Methods Peripheral blood lymphocytes separated from Sprague-Dawley(SD) male rats and healthy adults were exposed to γ-rays, and some rats were also subjected to total body irradiation. The inductions of DNA repair-related foci of γ-H2AX, pATM (S1981) and pDNA-PKcs (T2609) were detected with immunofluorescence staining technique at different time points post-irradiation, and the status of their co-localization was analyzed.Results The induction kinetics of γ-H2AX foci in rat lymphocytes was similar to that observed in human lymphocytes. The frequencies of γ-H2AX foci peaked at 30 min after γ-ray irradiation (trat=62.64, thuman=28.52,P<0.05), then decreased rapidly after 6 h post-irradiation (trat=45.96, thuman=14.80,P<0.05), and the residual foci number remained only about 3%-8% of its maximal value at 24 h post-irradiation. At 30 min after γ-ray irradiation,the frequencies of pATM (S1981) and pDNA-PKcs (T2609) foci in rat and human lymphocytes significantly higher than those of nonirradiated control (trat=21.05, 25.80, thuman=11.07, 29.52,P<0.05),and the frequencies of co-localization of pATM (S1981) or pDNA-PKcs (T2609) foci with γ-H2AX foci also markedly increased by 26%-32% in irradiated lymphocytes of rat and human (trat=5.34, 9.14, thuman=18.32, 51.28,P<0.05). Moreover, γ-H2AX foci incidence in rat lymphocytes in vitro was consistent with that induced by total body irradiation of rat. The number of γ-H2AX foci in irradiated rat lymphocytes increased with irradiation dose in a linear dose-dependent manner, its slope was similar to that of irradiated human lymphocytes reported by other laboratory. Conclusions Rat is a useful animal model to evaluate radiation biodosimetry with γ-H2AX foci in lymphocytes. The co-activation of ATM and DNA-PK plays an important role in DSB repair in the irradiated lymphocytes of rat and human. |
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