| Gao Hui,Zuo Siyao,Huang Zhengji,et al.Analysis of Th1-Th2-Th3 related gene expressions in the thymus of mice irradiated with different doses[J].Chinese Journal of Radiological Medicine and Protection,2015,35(4):248-251 |
| Analysis of Th1-Th2-Th3 related gene expressions in the thymus of mice irradiated with different doses |
| Received:August 29, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.04.003 |
| KeyWords:Radiation Dose Th cells PCR array Immune response |
| FundProject:国家自然科学基金(30870584,81371890);教育部高等学校博士学科点专项科研基金(20120061110063) |
| Author Name | Affiliation | E-mail | | Gao Hui | College of Public Medicine, Key Laboratory of Radiobiology, Ministry of Health, Jilin University, Changchun 130021, China | | | Zuo Siyao | College of Public Medicine, Key Laboratory of Radiobiology, Ministry of Health, Jilin University, Changchun 130021, China | | | Huang Zhengji | College of Public Medicine, Key Laboratory of Radiobiology, Ministry of Health, Jilin University, Changchun 130021, China | | | Han Hailing | College of Public Medicine, Key Laboratory of Radiobiology, Ministry of Health, Jilin University, Changchun 130021, China | | | Dong Juancong | College of Public Medicine, Key Laboratory of Radiobiology, Ministry of Health, Jilin University, Changchun 130021, China | | | Zhang Haiqin | College of Public Medicine, Key Laboratory of Radiobiology, Ministry of Health, Jilin University, Changchun 130021, China | | | Jin Shunzi | College of Public Medicine, Key Laboratory of Radiobiology, Ministry of Health, Jilin University, Changchun 130021, China | jinsz@jlu.edu.cn |
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| Abstract:: |
| Objective To analyze the effect of high and low dose radiation on the expressions of Th1, Th2 and Th3 /Tr1 related-genes in mice thymocytes and investigate the possible underlying molecular mechanism. Methods ICR mice were randomly divided into low-dose group (0.075 Gy), high-dose group (2.0 Gy) and sham-control group. The mouse thymus tissue was extracted at 16 hours after irradiation and the expressions of Th1-Th2-Th3 related genes were measured by PCR array. Results Eight genes were up-regulated and five genes were down-regulated after low dose radiation (0.075 Gy); while 54 genes were up-regulated and three genes were down-regulated after high dose (2.0 Gy) radiation. These genes included Th1 cell related genes, Th2 cell related genes, Th3/Tr1 cell related genes, Th1/Th2 immune response genes and transcription factor related genes. Low dose radiation induced up-regulation of Stat4 and Socs1 of genes related to the Th1 cells, and it induced down-regulation of IL-4ra, Cebpb, Gata3 and Tgfb3 associated with Th2 and Th3 cells, which lead to Sftpd genes up-regulation of Th1 immune response eventually. The high dose radiation up-regulated all of Th1, Th2 and Th3/Tr related genes and also enhanced the expressions of Cd86, IL-18, IL-10 and Irf4 genes related to Th2 immune response, but it did not alter the gene expression of Th1 immune response. Conclusions Low-dose radiation induces Th1-type immune response, while high doses radiation triggers Th2 type immune response. |
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