| Feng Jiangang,Li Shuang,Feng Jiangbin,Gao Ling,Lu Xue,Chen Deqing,Liu Qingjie.Dose-response of S100A4 gene expression in human lymphocytes at different time points after irradiation[J].Chinese Journal of Radiological Medicine and Protection,2015,35(4):245-247,302 |
| Dose-response of S100A4 gene expression in human lymphocytes at different time points after irradiation |
| Received:October 03, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.04.002 |
| KeyWords:Real-time PCR S100A4 Gene expression 60Co γ-rays |
| FundProject:国家自然科学基金(81172593) |
| Author Name | Affiliation | E-mail | | Feng Jiangang | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Li Shuang | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Feng Jiangbin | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Gao Ling | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Lu Xue | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Chen Deqing | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Liu Qingjie | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | qjliu@nirp.cn |
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| Abstract:: |
| Objective To investigate the dose response of S100A4 gene expression in the irradiated lymphoblastoid cells AHH-1 at different time points post irradiation. Methods AHH-1 cells was exposed to different doses(0, 1, 3, 5, 8, 10, 15 and 18 Gy)of 60Co γ-rays,and its mRNA levels of S100A4 was detected by reverse transcription PCR and real-time PCR at 4, 8, 12, 24, 48 and 72 h after irradiation. Results Within the range of applied doses, the level of S100A4 gene expression was up-regulated with a good dose-response (R2 =0.79-0.93, P<0.05)and had obvious difference at different time points (F=8.91, P<0.01). Conclusion S100A4 gene expression at transcriptional level could be detected easily and had optimum dose-responses at certain time points after irradiation, and hence is applicable as a dosimeter. |
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