| Shao Lihong,Jin Linlin,Zuo Siyao,et al.Effects of the microenvironment of irradiated tumor cells on Treg cells and related regulation factors[J].Chinese Journal of Radiological Medicine and Protection,2015,35(4):241-244 |
| Effects of the microenvironment of irradiated tumor cells on Treg cells and related regulation factors |
| Received:November 06, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.04.001 |
| KeyWords:Radiation A549 cell line Tumor microenvironment Regulatory T cells Immune tolerance |
| FundProject:国家自然科学基金(30870584,81371890);教育部高等学校博士学科点专项科研基金(20120061110063) |
| Author Name | Affiliation | E-mail | | Shao Lihong | College of Public Medicine, Key Laboratory of Radiobiology, Ministry of Health, Jilin University, Changchun 130021, China | | | Jin Linlin | College of Public Medicine, Key Laboratory of Radiobiology, Ministry of Health, Jilin University, Changchun 130021, China | | | Zuo Siyao | College of Public Medicine, Key Laboratory of Radiobiology, Ministry of Health, Jilin University, Changchun 130021, China | | | Wei Wei | College of Public Medicine, Key Laboratory of Radiobiology, Ministry of Health, Jilin University, Changchun 130021, China | | | Dong Zhuo | College of Public Medicine, Key Laboratory of Radiobiology, Ministry of Health, Jilin University, Changchun 130021, China | | | Zhang Haiqin | College of Public Medicine, Key Laboratory of Radiobiology, Ministry of Health, Jilin University, Changchun 130021, China | | | Han Hailing | College of Public Medicine, Key Laboratory of Radiobiology, Ministry of Health, Jilin University, Changchun 130021, China | | | Gao Hui | College of Public Medicine, Key Laboratory of Radiobiology, Ministry of Health, Jilin University, Changchun 130021, China | | | Jin Shunzi | College of Public Medicine, Key Laboratory of Radiobiology, Ministry of Health, Jilin University, Changchun 130021, China | jinsz@jlu.edu.cn |
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| Abstract:: |
| Objective To explore the immune tolerance in tumor microenvironment, we detected the expressions of CD receptors and related cytokines in Treg cells after being co-cultured with irradiated lung cancer cells. Methods Lymphocytes were isolated from human peripheral blood and cultured with lung adenocarcinoma cells A549, after irradiation with 6 Gy X-rays, the expression of CD4+CD25+, CD4+CD25+FoxP3+, CD4+CD25+NrP1+and ICOS, CTLA-4 in the lymphocytes were tested by flow cytometry,and the contents of IL-10, TGF-β and IL-17 in the supernatant were detected by ELISA. Results Compared with lymphocytes alone culture, the expressions of CD4+CD25+, CD4+CD25+FoxP3+, CD4+CD25+NrP1+, ICOS, CTLA-4, IL-10 and TGF-β in the co-culture groups with and without irradiation were all increased (t=2.78-40.61,P<0.05), the CD4+CD25+ and CTLA-4 in the lymphocytes were increased after irradiation (t=2.96,2.94,4.47,2.77,P<0.05). The content of IL-17 in the supernant were increased after co-cuture with irradiated A549 cells (t=4.00,4.71,P<0.05), but it had no obvious changes after irradiation alone. Conclusions The immune tolerance of lung cancer cells microenvironment under irradiation might contribute to the up-promotion of CD4+CD25+ Treg cells, CTLA-4 and IL-17 although the molecular mechanisms still need to be further investigated. |
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