| Zhou Ruiqing,He Huiqing,Guo Ziwen,Niu Xiaomin,Li Weihua,Qiu Dafa,Xu Xiaojun,Wang Jing.Effects of X-irradiation on the T-helper cell related cytokines in mouse[J].Chinese Journal of Radiological Medicine and Protection,2015,35(3):183-186 |
| Effects of X-irradiation on the T-helper cell related cytokines in mouse |
| Received:April 14, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.03.005 |
| KeyWords:X-rays T-helper cells Cytokines IFN-γ IL-17 |
| FundProject:广东省科技计划项目[粤科社字(2011)106号];中山市科技计划重大专项(20113A002);中山市科技计划(20122A111)资助项目 |
| Author Name | Affiliation | E-mail | | Zhou Ruiqing | Department of Hematology, Zhongshan Hospital of Sun Yat-Sen University, Guangdong 528403, China | | | He Huiqing | Department of Hematology, Zhongshan Hospital of Sun Yat-Sen University, Guangdong 528403, China | | | Guo Ziwen | Department of Hematology, Zhongshan Hospital of Sun Yat-Sen University, Guangdong 528403, China | | | Niu Xiaomin | Department of Hematology, Zhongshan Hospital of Sun Yat-Sen University, Guangdong 528403, China | | | Li Weihua | Department of Hematology, Zhongshan Hospital of Sun Yat-Sen University, Guangdong 528403, China | | | Qiu Dafa | Department of Hematology, Zhongshan Hospital of Sun Yat-Sen University, Guangdong 528403, China | | | Xu Xiaojun | Department of Hematology, Zhongshan Hospital of Sun Yat-Sen University, Guangdong 528403, China | doctorxu@163.com | | Wang Jing | 南方医科大学附属珠江医院血液内科 | |
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| Abstract:: |
| Objective To study the effects of X-ray irradiation at different doses on the expressions of the T-helper cell related cytokines in mouse.Methods 100 BALB/c mice were randomly divided into four groups (25 mice in each) with 0, 2, 4 and 6 Gy X-ray irradiation. At 1, 7, 14, 21 and 28 d after irradiation, the general situation of mice were observed, and the peripheral white blood cells were counted. The alterations of the serum helper T cell related cytokines were screened by protein chips and tested by ELISA.Results At 14 d after irradiation, the peripheral white blood cells of mice decreased to the lowest level. The number of peripheral white blood cells decrease along with the irradiation dose (Fdose=86.267,P<0.05). There were significant increases in the release of IFN-γ, TGF-β, L-6 and IL-17, but decreases in cytokine IL-5, IL-23 and TNF-α. ELISA analysis revealed that the increases of IL-17 correlated with the irradiation dose positively and the concentration of IL-17 in 6 Gy group were higher than control group at 1, 7, 14 and 21 d after irradiation (t=23.743, 21.759 and 17.662,P<0.05). The ratio of IFN-γ/IL-4 significantly higher than the control group at 1, 7, 14, 21 and 28 d after irradiation (t=8.335, 9.982, 6.990 and 3.074,P<0.05), and it approached to the highest level at 14 d and then began to decrease.Conclusions A low dose X-irradiation induces T-helper cell related cytokines IFN-γ and IL-17, and leads to Th1-biased immune responses in mice in vivo. |
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