| Xu Hualin,Sun Yang,Cui Fengmei,et al.MgSO4 inhibited X-rays irradiation induced expression of γ-H2AX in HUVEC[J].Chinese Journal of Radiological Medicine and Protection,2015,35(3):161-164 |
| MgSO4 inhibited X-rays irradiation induced expression of γ-H2AX in HUVEC |
| Received:September 23, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.03.001 |
| KeyWords:Magnesium sulfate Human umbilical vein endothelial X-rays CCK-8 γ-H2AX |
| FundProject:国家自然科学基金(81372921) |
| Author Name | Affiliation | E-mail | | Xu Hualin | School of Radiation Medicine and Protection, Medical School of Soochow University, Suzhou 215123, China | | | Sun Yang | School of Radiation Medicine and Protection, Medical School of Soochow University, Suzhou 215123, China | | | Cui Fengmei | School of Radiation Medicine and Protection, Medical School of Soochow University, Suzhou 215123, China | | | Tu Yu | School of Radiation Medicine and Protection, Medical School of Soochow University, Suzhou 215123, China | tuyu@suda.edu.com |
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| Abstract:: |
| Objective To investigate the influence of MgSO4 with different concentrations on cell survival and γ-H2AX expression in HUVEC irradiated with X-rays.Methods Cell proliferation rate was assayed by CCK-8, γ-H2AX foci formation was observed with a laser confocal microscope, and γ-H2AX protein expression was detected by flow cytometer and Western blot assay.Results MgSO4 with a concentration of 1.25 mg/ml could improve the survival rate of HUVEC treated with X-rays (t=-6.34,P<0.05).After irradiation, γ-H2AX foci approached to the highest level from 30 min to 1 h after radiation and then decreased. MgSO4 significantly reduced the foci formation at 0.5, 1, 2, 6 and 12 h post-irradiation (t=12.62, 6.36, 11.93, 5.75, 9.43,P<0.05). Both flow cytometry and Western blot assays showed that MgSO4 inhibited γ-H2AX protein expression at 0.5, 1 and 2 h post-irradiation (t=6.07, 5.32, 11.85,P<0.05).Conclusions MgSO4 could improve the survival rate and reduced γ-H2AX expression in HUVEC irradiated with X-ray. |
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