| Liu Hongyan,Dang Xuhong,Liu Jiangong,et al.Effects of 12C heavy-ion irradiation on cell growth, cell cycle and apoptosis of human peripheral blood lymphocytes[J].Chinese Journal of Radiological Medicine and Protection,2015,35(2):107-110 |
| Effects of 12C heavy-ion irradiation on cell growth, cell cycle and apoptosis of human peripheral blood lymphocytes |
| Received:May 20, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.02.007 |
| KeyWords:12C heavy-ions Human peripheral blood lymphocytes Cell proliferation Cell cycle Cell apoptosis |
| FundProject: |
| Author Name | Affiliation | E-mail | | Liu Hongyan | China Institute for Radiation Protection, Taiyuan 030006, China | | | Dang Xuhong | China Institute for Radiation Protection, Taiyuan 030006, China | | | Liu Jiangong | China Institute for Radiation Protection, Taiyuan 030006, China | | | Zhang Huifang | China Institute for Radiation Protection, Taiyuan 030006, China | | | Yuan Yayi | China Institute for Radiation Protection, Taiyuan 030006, China | | | Wang Chao | China Institute for Radiation Protection, Taiyuan 030006, China | | | Zuo Yahui | China Institute for Radiation Protection, Taiyuan 030006, China | | | Duan Zhikai | China Institute for Radiation Protection, Taiyuan 030006, China | duanzhikai@cirp.org.cn |
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| Abstract:: |
| Objective To explore the effects of 12C ions beam exposure on cell growth, cell cycle and apoptosis of lymphocytes.Methods Human peripheral blood lymphocytes Peng-EBV were exposed to 12C ions beams with different dose (0, 0.5, 2.0 Gy) at the dose rate of 0.3-0.5 Gy/min. The proliferation of Peng-EBV cells was determined by MTS assay. Cell apoptosis and cell cycle were analyzed by flow cytometry.Results The cellular proliferation was enhanced by 0.5 Gy irradiation but reduced by 2.0 Gy irradiation. The proliferation of irradiated cells could be recovered at 48 h after irradiation, but decreased at 72 h post irradiation. After 48 h of irradiation, G2/M cell cycle arrest was caused, especially at 2.0 Gy (t=2.01-99.80, P<0.05). At 30 d after irradiation, G2/M cell cycle arrest was not detected. At 12, 24, 48 h post-irradiation, apoptotic level was enhanced compared with the control (t=-3.05-1.05, P<0.05). The apoptosis rate had the highest level at 24 h post-irradiation, and then dropped at 48 h post-irradiation and recovered to control at 30 d post-irradiation.Conclusions The heavy-ion beam exposure could affect cellular proliferation, increase apoptosis and induce G2/M phase arrest of lymphocytes. |
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