| Yuan Yayi,Dang Xuhong,Zuo Yahui,Liu Hongyan,Zhang Ruifeng,Zhang Zhongxin,Ren Yue.Effects of CDT1 gene over-expression on cell apoptosis and cell cycle in irradiation-induced genomic instable liver cells[J].Chinese Journal of Radiological Medicine and Protection,2015,35(2):103-106 |
| Effects of CDT1 gene over-expression on cell apoptosis and cell cycle in irradiation-induced genomic instable liver cells |
| Received:July 09, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.02.006 |
| KeyWords:CDT1 Genomic instability Over-expression Cell cycle Cell apoptosis |
| FundProject: |
| Author Name | Affiliation | E-mail | | Yuan Yayi | China Institute for Radiation Protection, Taiyuan 030006, China | | | Dang Xuhong | China Institute for Radiation Protection, Taiyuan 030006, China | | | Zuo Yahui | China Institute for Radiation Protection, Taiyuan 030006, China | yahuiz@163.com | | Liu Hongyan | China Institute for Radiation Protection, Taiyuan 030006, China | | | Zhang Ruifeng | China Institute for Radiation Protection, Taiyuan 030006, China | | | Zhang Zhongxin | China Institute for Radiation Protection, Taiyuan 030006, China | | | Ren Yue | China Institute for Radiation Protection, Taiyuan 030006, China | |
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| Abstract:: |
| Objective To investigate the effect of CDT1 gene over-expression on the apoptosis and cell cycle distribution in liver cells with a characteristic of genomic instability induced by radiation(GIR).Methods Lentivirus particles were transferred into liver cells of GIR to up-regulate the expression of CDT1 gene. The apoptosis and the cell cycle were detected by flow cytometry (FCM). The expression changes of p53, ATM, ATR, Bcl-2, and Caspase-3 genes were analyzed by real-time fluorescence quantitative PCR.Results CDT1 gene was efficiently increased by the gene transfection(t=15.56, P<0.05). In the CDT1 over-expressed cells, while the apoptosis ratio was increased(t=4.19, P<0.05), the expressions of p53 and Bcl-2 gene were decreased (t=-4.21, -2.06, P<0.05), but the expression of ATM, ATR and Caspase-3 changed with no significant difference compared with control.Conclusions Over-expression of CDT1 could regulate genomic instability through apoptosis pathway and checkpoint independent of p53. |
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