| Wu Qinghua,He Yujie,Gu Chen,et al.Anti-tumor effect of 153Sm-DTPA-c(CGRRAGGSC) on nude mice model bearing MHCC97-H human liver cancer[J].Chinese Journal of Radiological Medicine and Protection,2015,35(2):92-97 |
| Anti-tumor effect of 153Sm-DTPA-c(CGRRAGGSC) on nude mice model bearing MHCC97-H human liver cancer |
| Received:June 18, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.02.004 |
| KeyWords:Liver cancer Interleukin-11 analogue 153Sm |
| FundProject:无锡市医院管理中心联合攻关项目(YGZX-1213) |
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| Abstract:: |
| Objective To observe the antitumor effect of 153Sm-DTPA-c(CGRRAGGSC) on MHCC97-H human liver cancer using a nude mice model.Methods 153Sm-DTPA-c(CGRRAGGSC) was indirectly synthesized by the reaction of 153SmCl3 with DTPA-c(CGRRAGGSC). Western blot was used to detect the expression changes of IL-11 receptor of eight different cell lines. 20 MHCC97-H tumor-bearing nude mice were randomly divided into four groups by random number table method with five mice in each group, administered with different activities of 153Sm-DTPA-c(CGRRAGGSC) by tail vein injection (5.5, 11.0, 22.0 MBq/0.2 ml and physiological saline/0.2 ml in turn). All nude mice were terminated after 16 d then the tumor tissue was observed and analyzed with microscopy, electron microscopy and immunohistochemistry for Ki-67, Bcl-2 and IL-11 receptor. Western blot was used to evaluate the expression changes on IL-11 receptor of tumor tissue at different doses.Results After administration with 153Sm-DTPA-c(CGRRAGGSC), the central region in the early stage of tumor became dry, necrosis, and crusting. In the late stage, tumor continually grew surrounding necrosis tissue. The inhibition rates in the low, middle and higher dose group were (22.72±2.76)%, (34.65±2.36)% and (85.13±5.78)%, respectively(F=89.32,P<0.05). The morphologic results showed that tumor structures were severely damaged along with radiation dose. The results of immunohistochemistry showed that the IL-11 receptor-positive cells in the control group and 5.5 MBq group was (84.13±5.71)% and (61.57±5.98)%(t=13.62,P<0.05), respectively. The Ki-67 and Bcl-2 positive cells were decreased in the injection group (t=20.91,6.68,P<0.05). Western blot analysis showed that the expression of IL-11 receptor protein decreased with antitumor-effect of radiation.Conclusions 153Sm-DTPA-c(CGRRAGGSC) effectively depresses the growth of MHCC97-H human liver cancer and suppresses the expression of IL-11 receptor, and hence could be a promoting agent for the treatment of MHCC97-H human liver cancer. |
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