| Ma Ya,Ding Butong,Lu Feng,Li Jieqing,Hou Dianjun,Jia Ximing,Qiao Jianwei,Li Weiguo.Analysis of Calyculin-A-induced prematurely condensed chromosome after high-dose irradiation by using fluorescence in situ hybridization (FISH)[J].Chinese Journal of Radiological Medicine and Protection,2015,35(2):88-91 |
| Analysis of Calyculin-A-induced prematurely condensed chromosome after high-dose irradiation by using fluorescence in situ hybridization (FISH) |
| Received:January 20, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.02.003 |
| KeyWords:Fluorescence in situ hybridization Prematurely condensed chromosome High-dose radiation |
| FundProject:山东省自然科学基金(ZR2010HQ061) |
| Author Name | Affiliation | | Ma Ya | Institute of Radiation Medicine, Shandong Academy of Medical Sciences, Jinan 250062, China | | Ding Butong | Institute of Radiation Medicine, Shandong Academy of Medical Sciences, Jinan 250062, China | | Lu Feng | Institute of Radiation Medicine, Shandong Academy of Medical Sciences, Jinan 250062, China | | Li Jieqing | Institute of Radiation Medicine, Shandong Academy of Medical Sciences, Jinan 250062, China | | Hou Dianjun | Institute of Radiation Medicine, Shandong Academy of Medical Sciences, Jinan 250062, China | | Jia Ximing | Institute of Radiation Medicine, Shandong Academy of Medical Sciences, Jinan 250062, China | | Qiao Jianwei | Institute of Radiation Medicine, Shandong Academy of Medical Sciences, Jinan 250062, China | | Li Weiguo | Institute of Radiation Medicine, Shandong Academy of Medical Sciences, Jinan 250062, China |
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| Abstract:: |
| Objective To explore the feasibility of using fluorescence in situ hybridization (FISH) technology to detect the Calyculin A (CA)-induced prematurely condensed chromosome(PCC) after high-dose irradiation of X-rays.Methods Human peripheral blood was irradiated by X-rays at 0, 1, 5,10, 15 and 20 Gy. Whole blood was cultured with RPMI 1640 medium, thereafter the CA was used to induce premature chromosome condensation. The No.1 and No.4 whole chromosome probe was used in the FISH, and the chromosomes were observed under the fluorescence microscope. The aberration positive cell quantity and the quantity of fragments of two chromosomes were counted, and dose-effect curve fitted was performed.Results The ratio of chromosomal aberration positive cells had a good dose response (0-15 Gy) with an equation Y=0.008+0.065D+1.858×10-5D2(R2=0.994). The PCCfragments also had a good dose response within 0-20 Gy with an equation Y=-0.032+0.216D-0.01D2(R2=1.0).Conclusions Analysis of Calyculin-A-induced PCC by using FISH technique could be used to estimate the biological dose after high-dose irradiation. |
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