| Xu Chang,Wang Yan,Du Liqing,Wang Qin,Liu Qiang.Effect and mechanism of neuroepithelial cell transforming gene 1 on cellular radiosensitivity[J].Chinese Journal of Radiological Medicine and Protection,2015,35(1):49-52 |
| Effect and mechanism of neuroepithelial cell transforming gene 1 on cellular radiosensitivity |
| Received:October 14, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.01.009 |
| KeyWords:Net1 Radiosensitivity DNA double-strand break Non-homologous end joining |
| FundProject:国家自然科学基金(31200634, 31170804);天津市自然科学基金(12JCYBJC32900) |
| Author Name | Affiliation | E-mail | | Xu Chang | Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Sciences, Tianjin 300192, China | | | Wang Yan | Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Sciences, Tianjin 300192, China | | | Du Liqing | Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Sciences, Tianjin 300192, China | | | Wang Qin | Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Sciences, Tianjin 300192, China | | | Liu Qiang | Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Sciences, Tianjin 300192, China | liuqiang@irm-cams.ac.cn |
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| Abstract:: |
| Objective To study the effect of neuroepithelial cell transforming gene 1(Net1) on the cellular radiosensitivity and underlying mechanism.MethodsReal-time quantitative PCR was used to measure the variations in Net1 expression level upon irradiation. Radiosensitivity was analyzed by colony-forming assay after Net1-siRNAs. Net1-associated proteins were identified by co-immunoprecipitation. Results The Net1 mRNA level in the cells was increased significantly (t=－10.52, P<0.05) after irradiation. Compared to the control group, siRNA-mediated silencing of Net1 enhanced cell radiosensitivity (t=15.31, 11.65, P<0.05). Net1 was found to interact with Ku70, Ku80 and DNA-PKcs under either normal conditions or after irradiation. Conclusions Net1 could protect cells from irradiation by interaction with DNA repair proteins in non-homologous end joining pathway. |
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