| Zhang Chaoxiong,Zhang Mingjian,Gao Fu,Zhou Chuanfeng,Zhang Pei,Cai Jianming,Liu Cong.Down regulation of miR-203 in radiation- induced thymic lymphoma promoted cells proliferation and inhibited apoptosis[J].Chinese Journal of Radiological Medicine and Protection,2015,35(1):28-34 |
| Down regulation of miR-203 in radiation- induced thymic lymphoma promoted cells proliferation and inhibited apoptosis |
| Received:September 01, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.01.005 |
| KeyWords:MiR-203 Radiation-induced thymic lymphoma (RITL) TANK-binding kinase 1 (TBK1) SLUG (SNAI2) Cyclin D1 (CCND1) |
| FundProject:This work was supported by grant from National Natural Science Foundation of China (81402630). |
| Author Name | Affiliation | E-mail | | Zhang Chaoxiong | Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433 | | | Zhang Mingjian | Xingcheng Sanatorium of Shenyang Military Region | | | Gao Fu | Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433 | | | Zhou Chuanfeng | Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433 | | | Zhang Pei | Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433 | | | Cai Jianming | Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433 | | | Liu Cong | Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433 | victorliu20102020@163.com |
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| Abstract:: |
| Objective To investigate the role of miR-203 in radiation-induced thymic lymphoma (RITL).Methods A 60Co irradiator was used for total-body irradiation. MicroRNAs(miRNAs) level was assayed by qRT-PCR. Cell proliferation was assayed by MTT assay. Cell apoptosis was examined by fluorescence activated cell sorter(FACS). Dual luciferase reporter assay system was used to detect the 3'UTR reporter. Results MiR-203 was down-regulated in RITL tissues. Overexpression of miR-203 strongly inhibited the proliferation of both NIH3T3 cells and EL4 cells and vice versa. MiR-203 inhibited cells proliferation and induced apoptosis via TANK-binding kinase (TBK1), SLUG(SNAI2) and Cyclin D1(CCND1). Conclusions Radiation down-regulated the level of miR-203 in thymic, which promoted radiation-induced thymic lymphoma by targeting TBK1, SNAI2 and CCND1. |
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