| Wu Liao,Tan Li,Wang Yu,Liu Dengqun,Shi Chunmeng.Engraftment of bone marrow-derived cells after nonlethal radiation in syngeneic C57BL/6 mice[J].Chinese Journal of Radiological Medicine and Protection,2015,35(1):23-27 |
| Engraftment of bone marrow-derived cells after nonlethal radiation in syngeneic C57BL/6 mice |
| Received:October 28, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.01.004 |
| KeyWords:Chimerism Bone marrow-derived cells Nonlethal radiation |
| FundProject:This work was supported by grants from State Key Basic Research Development Program (2012CB518103), Program of New Century Excellent Talents in University (NCET-11-0869) from Ministry of Education, Innovation Team Building Program of Chongqing University (KJTD201338),and Youth Innovation Foundation of Third Military Medical University Grant(2010XQN12). |
| Author Name | Affiliation | E-mail | | Wu Liao | Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038 | | | Tan Li | Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038 | | | Wang Yu | Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038 | | | Liu Dengqun | Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038 | | | Shi Chunmeng | Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038 | shicm@sina.com |
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| Abstract:: |
| Objective To study the characteristics of cell engraftment in mice at a lower dose under nonlethal radiated condition.Methods A syngeneic C57BL/6 mouse model, transplanted with 1×107 bone marrow cells and exposed to 2.5 Gy whole body irradiation (WBI), was selected to study the chimerism of cells from green fluorescent protein positive (GFP+) transgenic mice. The control group was injected with GFP+ cells without receiving irradiation. In addition, an allogenic transplantation model of BALB/c mice was also investigated which was infused by GFP+ cells from C57BL/6 mice. The engraftment of bone marrow-derived cells (BMDCs) was detected by immunohistochemistry in bone marrow, liver, lung, small intestine and spleen. Results The transplanted bone marrow cells successfully grafted in the haematopoietic tissues from syngeneic GFP transgenic mice. The transplanted GFP+ cells were also detected in the non-haematopoietic tissues, such as the small intestine, liver, spleen and lung, after irradiation. However, a lethal dose irradiation of 8 Gy was required to establish successful chimerism in allogeneic transplantation model by infusing the bone marrow cells from C57BL/6 mice to BALB/c mice. Conclusions Bone marrow-derived cells can be successfully grafted into various recipient tissues receiving a 2.5 Gy dose of radiation in syngeneic mice, but not in allogeneic mice. This nonlethal model may help to further study the plasticity and mechanism of bone marrow-derived cells in tissue repair and regeneration after radiation injury. |
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