| Yu Yang,Cao Huiling,Xu Ye,Liu Shibing.The mechanism of non-thermal effect of 2.45 GHz microwave on HeLa cell apoptosis[J].Chinese Journal of Radiological Medicine and Protection,2014,34(12):908-911 |
| The mechanism of non-thermal effect of 2.45 GHz microwave on HeLa cell apoptosis |
| Received:June 10, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.12.007 |
| KeyWords:Microwave Non-thermal effect HeLa cells Cell apoptosis |
| FundProject:吉林省教育厅"十一五"科学技术研究(吉教科合字[2010]第253号) |
| Author Name | Affiliation | E-mail | | Yu Yang | Medical Research Laboratory, Jilin Medical College, Jilin 132013, China | | | Cao Huiling | Medical Research Laboratory, Jilin Medical College, Jilin 132013, China | 179265336@qq.com | | Xu Ye | Medical Research Laboratory, Jilin Medical College, Jilin 132013, China | | | Liu Shibing | Medical Research Laboratory, Jilin Medical College, Jilin 132013, China | |
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| Abstract:: |
| Objective To investigate the effects of 2.45 GHz microwave on the apoptosis of HeLa cells and explore the underlying mechanism.Methods HeLa cells were divided into five groups randomly: control group, sham-irradiation group, and three irradiation groups exposed with 10,15 and 20 mW/cm2 2.45 GHz microwave for 10 min. Cell survival was tested by MTT assay, cell nucleus fashion was observed by a laser scanning confocal microscope, cell cycle distribution was measured by flow cytometry, and cell protein expressions were detected by Western blot. Results The cell survival in control group, sham-irradiation group and low dose irradiation group had no difference but it became lower in the middle and high dose irradiated group(t=-16.47, -14.23, P<0.05). The abnormal rates of nuclear fashion in the low,middle and high dose irradiation groups were obviously higher compared with control group(t=9.37, 11.25, 8.47, P<0.05), while high dose irradiation group was higher than the low and middle dose group (t=15.32, 10.25, P<0.05). The rate of HeLa cells in sub-G1 in the irradiation groups were higher than that in control group(t=15.24, 22.31, 10.69, P<0.05). The results of immunohistochemistry showed that the expression of Bax and Caspase-3 in irradiation groups was significantly increased, and the expression of Bcl-2 was decreased. Conclusions 2.45 GHz microwave can induce HeLa cells apoptosis with a positive correlation with microwave dose. |
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