| Gao Ling,Liu Qiaowei,Li Fengsheng,Chen Xiaohua,Feng Jiangbin,Lu Xue,Chen Deqing,Su Xu.Radiation-induced activation of STAT3 in pulmonary adenocarcinoma[J].Chinese Journal of Radiological Medicine and Protection,2014,34(12):900-903 |
| Radiation-induced activation of STAT3 in pulmonary adenocarcinoma |
| Received:April 16, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.12.005 |
| KeyWords:Adenocarcinma Irradiation p-STAT3 EGFR |
| FundProject:国家自然基金科学部主任基金(31340051);国家自然科学基金青年科学基金(81001216);全军医学科技"十二五"科研项目(CWS12J082) |
| Author Name | Affiliation | E-mail | | Gao Ling | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Liu Qiaowei | 军事医学科学院放射与辐射医学研究所 | | | Li Fengsheng | 第二炮兵总医院 全军核辐射损伤医学监测与防治研究中心 | | | Chen Xiaohua | 军事医学科学院放射与辐射医学研究所 | | | Feng Jiangbin | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Lu Xue | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Chen Deqing | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Su Xu | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | suxu@nirp.cn |
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| Abstract:: |
| Objective To investigate the dose-, time-effect of STAT3 activation induced by radiation and explore the underlying signal pathway. Methods Pulmonary adenocarcinoma cells (H322) with mutated p53 gene were irradiated with 0-10 Gy of γ-rays. After radiation, the expression and transcriptional activity of STAT3 and the levels of its upstream molecules, including p-EGFR, were detected by Western blot. The nucleus translocation of p-STAT3 was examined by immunofluorescence staining assay. Results The protein expression and transcriptional activation of p-STAT3 in H322 cells were increased by 1.9-6.6 fold after 2-10 Gy radiation and increased by 1.2-9.5 fold after 0-24 h of irradiation. In addition, the expression of p-EGFR was also increased by 4.3-19.2 fold after irradiation. Conclusions After radiation, the expression of p-STAT3 in the pulmonary adenocarcinoma H322 cell can be increased probably through EGFR/STAT3 signal pathway. |
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