| Guo Siyan,Zhu Xiaodong,Ge Lianying,Qu Song,Li Ling,Guo Ya,Liang Shixiong,Su Fang.Effect of c-jun gene RNAi on the radiosensitivity of radioresistant human nasopharyngeal carcinoma cell line CNE-2R[J].Chinese Journal of Radiological Medicine and Protection,2014,34(12):895-899 |
| Effect of c-jun gene RNAi on the radiosensitivity of radioresistant human nasopharyngeal carcinoma cell line CNE-2R |
| Received:June 04, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.12.004 |
| KeyWords:Nasopharyngeal carcinoma Radioresistance c-jun RNA interference |
| FundProject:国家自然科学基金(30860329);广西壮族自治区自然科学基金(桂科自0832229);广西高等学校重大科研资助项目(201101ZD004) |
| Author Name | Affiliation | E-mail | | Guo Siyan | Department of Radiotherapy, Cancer Hospital of Guangxi Medical University & Cancer Institute of Guangxi Zhuang Autonomous Region, Key Laboratory of High-Incidence-Tumor Prevention & Treatment(Guangxi Medical University), Ministry of Education, Nanning 530021, China | | | Zhu Xiaodong | Department of Radiotherapy, Cancer Hospital of Guangxi Medical University & Cancer Institute of Guangxi Zhuang Autonomous Region, Key Laboratory of High-Incidence-Tumor Prevention & Treatment(Guangxi Medical University), Ministry of Education, Nanning 530021, China | zhuxiaodong83@163.com | | Ge Lianying | Department of Radiotherapy, Cancer Hospital of Guangxi Medical University & Cancer Institute of Guangxi Zhuang Autonomous Region, Key Laboratory of High-Incidence-Tumor Prevention & Treatment(Guangxi Medical University), Ministry of Education, Nanning 530021, China | | | Qu Song | Department of Radiotherapy, Cancer Hospital of Guangxi Medical University & Cancer Institute of Guangxi Zhuang Autonomous Region, Key Laboratory of High-Incidence-Tumor Prevention & Treatment(Guangxi Medical University), Ministry of Education, Nanning 530021, China | | | Li Ling | Department of Radiotherapy, Cancer Hospital of Guangxi Medical University & Cancer Institute of Guangxi Zhuang Autonomous Region, Key Laboratory of High-Incidence-Tumor Prevention & Treatment(Guangxi Medical University), Ministry of Education, Nanning 530021, China | | | Guo Ya | 西安交通大学第二附属医院肿瘤科 | | | Liang Shixiong | Department of Radiotherapy, Cancer Hospital of Guangxi Medical University & Cancer Institute of Guangxi Zhuang Autonomous Region, Key Laboratory of High-Incidence-Tumor Prevention & Treatment(Guangxi Medical University), Ministry of Education, Nanning 530021, China | | | Su Fang | Department of Radiotherapy, Cancer Hospital of Guangxi Medical University & Cancer Institute of Guangxi Zhuang Autonomous Region, Key Laboratory of High-Incidence-Tumor Prevention & Treatment(Guangxi Medical University), Ministry of Education, Nanning 530021, China | |
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| Abstract:: |
| Objective To investigate the effect of c-jun RNAi on the radiosensitivity of radioresistant human nasopharyngeal carcinoma cell line CNE-2R and the possible mechanisms. Methods Three siRNA sequences specific for c-jun gene were designed, and lentvirus vector with c-jun shRNA was constructed and infected into CNE-2R. The gene silencing efficiency of these recombinants was detected by RT-PCR and Western blot. Radiosensitivity, cell proliferation and apoptosis were measured using colony formation assay, CCK-8 assay and flow cytometry respectively. Results Recombinant lentiviral c-jun-RNAi-LV2 obviously inhibited c-jun expression at mRNA and protein level (F=217.20, 42.07, P<0.05). The cell proliferation of combination group (c-jun-RNAi-LV2 plus 0, 2, 4, 6, 8 Gy of 6 MV X-rays) was significantly lower than that of the other two groups (F=42.70-200.67, P<0.05). The cloning formation of c-jun-RNAi-LV2 group was significantly lower than that of non-transfected group and NC-transfected group and its sensitizing enhance rate (SER) at D0 was 1.41. The apoptosis rates of 0, 2 Gy X-ray radiation in c-jun-RNAi-LV2 group, non-transfected group and NC-transfected group were(20.93±1.99)%, (38.17±0.83)%;(10.97±0.70)%, (20.43±0.25)%;(10.80±1.25)% and (19.53±1.50)% (F=50.54, 330.14, P<0.05), respectively. Conclusions RNA interference inhibited the c-jun expression and enhanced the radiation sensitivity of human nasopharyngeal carcinoma cell line CNE-2R, perhaps due to that the c-jun-RNAi-LV2 mediated c-jun down-regulation inhibited cell proliferation and promoted cell apoptosis. |
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