| Cheng Yizhuang,Yao Xiaobo,Wu Cuiping,Wang Yongsheng,Chen Ke,Zhang Wukui,Wang Mingming.Effects of 18F-FDG on P53, XIAP, and GADD45 expressions in transplanted Lewis lung carcinoma in mice[J].Chinese Journal of Radiological Medicine and Protection,2014,34(12):884-888 |
| Effects of 18F-FDG on P53, XIAP, and GADD45 expressions in transplanted Lewis lung carcinoma in mice |
| Received:March 28, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.12.002 |
| KeyWords:Lewis lung carcinoma P53 protein XIAP protein GADD45 protein |
| FundProject:国家自然科学基金(81273003);安徽省自然科学基金(1208085MH162);安徽省科技攻关项目(10021303035);安徽省教育厅自然科学基金(KJ2011Z162) |
| Author Name | Affiliation | E-mail | | Cheng Yizhuang | Department of Nuclear Medicine, Anhui Provincial Hospital, Hefei 230001, China | | | Yao Xiaobo | Department of Nuclear Medicine, Anhui Provincial Hospital, Hefei 230001, China | | | Wu Cuiping | 安徽医科大学临床医学院放射医学研究所, 合肥 230001 | | | Wang Yongsheng | 安徽医科大学临床医学院放射医学研究所, 合肥 230001 | | | Chen Ke | Department of Nuclear Medicine, Anhui Provincial Hospital, Hefei 230001, China | | | Zhang Wukui | 安徽医科大学临床医学院放射医学研究所, 合肥 230001 | | | Wang Mingming | 安徽医科大学临床医学院放射医学研究所, 合肥 230001 | wmgene@163.com |
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| Abstract:: |
| Objective To study the effects of internal exposure of 18F-FDG (18F-2-deoxy-2-fluoro-D-glucose) on the protein expressions of P53, XIAP (X-linked inhibitors of apoptosis protein) and GADD (growth arrest and DNA damage)45 in Lewis lung carcinoma, and to explore the possibility of applying 18F-FDG as a radiotherapy drug in vivo. Methods Lewis lung cancer transplanted tumor models were established via subcutaneous injection of 0.2 ml of 2×107/ml Lewis lung carcinoma cells at left hind limbs of 48 C57BL/6 mice that were randomly divided into high dose group, low dose group and control group with 16 mice each. After 7 d of cancer cell inoculation, 18.5×107 Bq and 9.25×107 Bq of 18F-FDG in 0.2 ml saline or equal volume of physiological saline was intraperitoneally injected into three group mice, respectively. 22 d post inoculation, the protein expressions of P53, XIAP and GADD45 were immuo-histochemically detected by using SP approach. Results There was significant difference among the protein expressions in each group (χ2=8.30, 16.02, 7.68, P<0.05). The mean integral optic density of protein expression increased from 0.089±0.033 in control group to 0.315±0.028 in high dose group for P53, and from 0.126±0.023 to 0.383±0.035 for GADD45, but it decreased from 0.422±0.034 to 0.149±0.055 for XIAP. There was significantly difference of these protein expressions in each group (P53:F=5.26,P<0.05;XIAP: F=4.29,P<0.05;GADD45:F=5.98,P<0.05). Conclusions 18F-FDG can up-regulate the expressions of P53 and GADD45 proteins and down-regulate the expression of XIAP protein in tumor cells, and it inhibits tumor growth by promoting cell apoptosis in the Lewis lung carcinoma tissue with a P53-dependent manner. |
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