| He Ying,Shen Xianrong,Wang Qingrong,Jiang Dingwen,Hou Dengyong,Liu Yuming,Chen Wei,Li Kexian,Liu Qiong,Qian Tiantian.Effects of low dose γ-ray on protein expression of human lymphoblastoid cells[J].Chinese Journal of Radiological Medicine and Protection,2014,34(11):832-836 |
| Effects of low dose γ-ray on protein expression of human lymphoblastoid cells |
| Received:October 29, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.11.008 |
| KeyWords:Low-dose Two-dimensional electrophoresis Real-time PCR Eukaryotic translation initiation factor 5A Fatty acid binding protein 4 |
| FundProject:全军计划科研项目(AHJ09J012);总后"十一五"科技攻关项目(2009183006) |
| Author Name | Affiliation | E-mail | | He Ying | Naval Medical Research Institute of PLA, Shanghai 200433, China | | | Shen Xianrong | Naval Medical Research Institute of PLA, Shanghai 200433, China | xianrong_sh@163.com | | Wang Qingrong | Naval Medical Research Institute of PLA, Shanghai 200433, China | | | Jiang Dingwen | Naval Medical Research Institute of PLA, Shanghai 200433, China | | | Hou Dengyong | Naval Medical Research Institute of PLA, Shanghai 200433, China | | | Liu Yuming | Naval Medical Research Institute of PLA, Shanghai 200433, China | | | Chen Wei | Naval Medical Research Institute of PLA, Shanghai 200433, China | | | Li Kexian | Naval Medical Research Institute of PLA, Shanghai 200433, China | | | Liu Qiong | Naval Medical Research Institute of PLA, Shanghai 200433, China | | | Qian Tiantian | Naval Medical Research Institute of PLA, Shanghai 200433, China | |
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| Abstract:: |
| Objective To observe the alterations of protein expression in human normal lymphoblastoid cell AHH-1 lines, after irradiation with different doses of γ-rays, in order to investigate mechanisms of biological effects induced by low dose radiation. Methods Two-dimensional electrophoresis (2-DE) was performed and differential expression proteins were identified by matrix assisted laser desorption/ionization time of flight mass spectrometry (MAIDI-TOF-MS) after irradiation. The mRNA expressions of proteins were analyzed by real-time quantity PCR (RTQ-PCR). Results Using 2-DE and MAIDI-TOF-MS, 3 kinds of altered proteins were definite in terms of eukaryotic translation initiation factor 5A (eIF5A), profoldin subunit 1(PFDN1) and fatty acid binding protein 4 (FABP4). The mRNA expressions of eIF5A and FABP4 were coincided with protein expressions. Conclusions These differentially expressed proteins induced by low-doses of γ-rays will help to elucidate the mechanisms of biological effects at low doses and offer the reference for further research of novel biomarkers for low dose exposure. |
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