| Yuan Xiaoli,Li Tao,Huang Jianming,Zha Xiao,Deng Bifang,Lang Jinyi.Study of radiosensitization of chloroquine on esophageal cancer cell line[J].Chinese Journal of Radiological Medicine and Protection,2014,34(11):823-826 |
| Study of radiosensitization of chloroquine on esophageal cancer cell line |
| Received:November 10, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.11.006 |
| KeyWords:Esophageal neoplasms Autophagy Chloroquine Radiosensitivity |
| FundProject:四川省科技厅科技支撑项目(2012SZ0081) |
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| Abstract:: |
| Objective To investigate the possibility of chloroquine radiosensitization of esophageal cancer cell line TE-1 and its further mechanism.Methods Effect of chloroquine on cell viability of TE-1 cells was determined by MTT method. Expression of LC3, Beclin-1 and formation of acidic vesicular organelles (AVOs) were determined by Western blot, and fluorescence staining with Lyso-Tracker Red DND-99, respectively. Clonogenic survival of TE-1 cells was examined by clonogenic forming assay. Results Chloroquine showed dose-dependent inhibition of TE-1 cell growth, and its values of IC50 and IC10 were (72.33±5.28) and (15.42±3.33) μmol/L, respectively. The expression of Beclin-1 and LC3-II/I markedly increased in irradiated TE-1 cells. The addition of chloroquine with IC10concentration significantly reduced the fluorescence and intensity of AVOs accumulation in the cytoplasm of TE-1 cells. Clonogenic survival fraction decreased obviously in TE-1 cells with addition of chloroquine after radiation and the value of SERD0 was 1.439. Conclusions Chloroquine could radiosensitize esophageal cancer cells by blocking autophagy-lysosomal pathway and be used as a potential radiosensitizing strategy. |
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