| Zhou Meijuan,Huang Haibo,Lin Zhixiang,Ding Zhenhua.The carcinogenic effect of UVB sensitive miR-365 in cutaneous squamous cell carcinoma[J].Chinese Journal of Radiological Medicine and Protection,2014,34(11):813-816,866 |
| The carcinogenic effect of UVB sensitive miR-365 in cutaneous squamous cell carcinoma |
| Received:April 30, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.11.004 |
| KeyWords:MiR-365 UVB Cutaneous squamous cell carcinoma Oncogene |
| FundProject:国家自然科学基金(81172634,81202152) |
| Author Name | Affiliation | E-mail | | Zhou Meijuan | Department of Radiation Medicine, School of Public Health and Tropic Medicine, Southern Medical University, Guangzhou 510515, China | | | Huang Haibo | Department of Radiation Medicine, School of Public Health and Tropic Medicine, Southern Medical University, Guangzhou 510515, China | | | Lin Zhixiang | Department of Radiation Medicine, School of Public Health and Tropic Medicine, Southern Medical University, Guangzhou 510515, China | | | Ding Zhenhua | Department of Radiation Medicine, School of Public Health and Tropic Medicine, Southern Medical University, Guangzhou 510515, China | dingzh@smu.edu.cn |
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| Abstract:: |
| Objective To investigate the carcinogeic role of miR-365 in cuntanerous squamous cell carcinoma (cSCC). Methods Normal HaCaT cells were divided into control and irradiation groups, the later was exposed by UVB irradiation (50 J/m2). MicroRNA expression profiles of the two groups were analyzed by microRNA array. The expression variations of miR-365 in HaCaT, A431, Tca8113 and HSC-1 cells were validated by qRT-PCR analysis. The colony-forming and invasion capacities were dectected by colony forming assay and Transwell migration assay in vitro, respectively. HaCaTpre-miR-365-2 highly expressing miR-365 was constructed by retroviral vector infection. Tumorigenicity evaluation was carried out by subcutaneously inject of the cells at the right back flank of nude mice. Results There were 30 microRNAs differentially expressed in HaCaT cells after UVB irradiation and miR-365 was one of the most sensitive miRNAs(as high 6.7 times as control). Expression of miR-365 in all the cSCC cell lines A431, Tca8113 and HSC-1 were significantly higher than that in HaCaT cell, in which the maximum was A431 (15.67±1.12 times,P<0.01), and the minimum was Tca8113(4.72±0.85 times,P<0.05). Knockdown of miR-365 in cSCC cell lines significantly inhibited the colony forming ability (t=13.68,P<0.05) and cell migration (t=19.98,P<0.05) in vitro. HaCaT cells overexpressing miR-365 by transient transfection significantly increased the ability of colony formation (t=7.11,P<0.05) and cell migration (t=22.03,P<0.05) in vitro. In addition, HaCaTpre-miR-365-2 cell line stably expressing miR-365 could successfully establish tumors in nude mice. Conclusions MiR-365 is an oncogene for cutaneous squamous cell carcinoma. |
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