| Dong Juancong,Zhang Haiqin,Gao Hui,Han Hailing,Jin Linlin,Shao Lihong,Wei Wei,Jin Shunzi.Effect of neuropilin-1 on radio-sensitivity of non-small cell lung carcinoma[J].Chinese Journal of Radiological Medicine and Protection,2014,34(11):809-812 |
| Effect of neuropilin-1 on radio-sensitivity of non-small cell lung carcinoma |
| Received:April 25, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.11.003 |
| KeyWords:Neuropilin 1 Non-small cell lung carcinoma A549 cell Radiosensitivity Radioresistance |
| FundProject:国家自然科学基金(30870584,81371890);教育部高等学校博士学科点专项科研基金(20120061110063) |
| Author Name | Affiliation | E-mail | | Dong Juancong | China Institute for Radiation Protection, Taiyuan 030006, China | | | Zhang Haiqin | College of Public Medicine, Key Laboratory of Radiobiology, Ministry of Health, Jilin University, Changchun 130021, China | | | Gao Hui | College of Public Medicine, Key Laboratory of Radiobiology, Ministry of Health, Jilin University, Changchun 130021, China | | | Han Hailing | College of Public Medicine, Key Laboratory of Radiobiology, Ministry of Health, Jilin University, Changchun 130021, China | | | Jin Linlin | College of Public Medicine, Key Laboratory of Radiobiology, Ministry of Health, Jilin University, Changchun 130021, China | | | Shao Lihong | College of Public Medicine, Key Laboratory of Radiobiology, Ministry of Health, Jilin University, Changchun 130021, China | | | Wei Wei | College of Public Medicine, Key Laboratory of Radiobiology, Ministry of Health, Jilin University, Changchun 130021, China | | | Jin Shunzi | College of Public Medicine, Key Laboratory of Radiobiology, Ministry of Health, Jilin University, Changchun 130021, China | jinsz@jlu.edu.cn |
|
| Hits: 3031 |
| Download times: 2222 |
| Abstract:: |
| Objective To investigate the expression and relationship with radiosensitivity of neuropilin-1 (NRP-1) of non-small cell lung carcinoma (NSCLC) cells. Methods The basal expression level of NRP1 in the cells of H358, H460, H1299, A549 and SK-MES-1were detected by Western blot. MTT method was used to measure the apoptotic rate, and two kinds of cells were selected for further study on radiosensitivity and radioresistance. The surviving fraction (SF2) and apoptosis rate were analyzed with clonogenic assay and flow cytometry respectively. Results The expression level from high to low of NRP1 in order was: A549>SK-MES-1>H358>H460>H1299, while the radiosensitivity from strong to weak was H460>H1299>H358>SK-MES-1>A549. Therefore, A549 and H460 cells were chosen to do follow-up studies. The SF was 0.887 at 2 Gy in A549 cells,while 0.313 in H460 cells. After 10 Gy X-rays, the apoptosis rate of H460 cells was 238.88%, significantly higher than that of A549 cells (122.54%). Ionizing radiation induced up-regulation of NRP1 expression in A549 cells by up to 40%, while the targeted inhibition of NRP1 enhanced the radiosensitivity of A549 cells. Conclusions A549 cells were more radioresistance than other cells, which may be associated with the up-regulation of NRP1 expression. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
