| Wang Yunlian,Ailijiang·Tuerxun,Ainiwaer·Aimudula,et al.Expression of CTGF in radiation-induced hepatic fibrosis and its relationship with hepatic stellate cell activation in rats[J].Chinese Journal of Radiological Medicine and Protection,2014,34(10):748-752 |
| Expression of CTGF in radiation-induced hepatic fibrosis and its relationship with hepatic stellate cell activation in rats |
| Received:March 20, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.10.007 |
| KeyWords:CTGT TGF-β1 α-SMA Hepatic fibrosis Radiation |
| FundProject:新疆维吾尔自治区自然科学基金（2012211A077） |
| Author Name | Affiliation | E-mail | | Wang Yunlian | Department of Oncology, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China | | | Ailijiang·Tuerxun | Department of Oncology, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China | | | Ainiwaer·Aimudula | Department of Oncology, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China | | | Xiao Lei | Department of Oncology, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China | | | Zhang Yuefen | Department of Oncology, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China | | | Yang Ying | Department of Oncology, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China | | | Wu Ge | Department of Oncology, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China | | | Cheng Wei | Department of Oncology, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China | | | Bao Yongxing | Department of Oncology, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China | baoyx@vip.sina.com |
|
| Hits: 3678 |
| Download times: 2802 |
| Abstract:: |
| Objective: To study the expression of connective tissue growth factor (CTGF) in radiation-induced hepatic fibrosis and explore its correlation with the expression of transforming growth factor β1(TGF-β1) and α-smooth muscle actin (α-SMA) as well as the activation of hepatic stellate cells (HSC). Methods: Forty healthy male Sprague-Dawley rats were randomly divided into model group (30 rats) and control group (10 rats). The right-half liver of rat was irradiated with a single dose of 25 Gy to establish a radiation-induced hepatic fibrosis model. Liver tissues of irradiated rats were collected at 2, 4, and 6 months after irradiation. Pathomorphological changes in these liver tissues were stained with hematoxylin-eosin and observed with an optical microscope. The mRNA and protein expressions of CTGF, TGF-β1 and α-SMA were assayed by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. Results: The longer the irradiation time, the degree of liver fibrosis was more serious. The expressions of CTGF, TGF-β1, α-SMA protein in the model group increased along with the time after irradiation (F=55.44, 16.22, 121.12, P<0.05). The mRNA expressions of CTGF, TGF-β1 and α-SMA in the model group had positive correlations (r=0.638, 0.715, P<0.05) and were significantly higher than the control (F=177.11, 133.85, 217.46, P<0.05). Conclusions: The expression of CTGF had a positive relationship with the development of hepatic fibrosis. CTGF may be involved in the activation of TGF-β signaling pathway and thereby promotes the activation and proliferation of HSC. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
