| Zhang Zhubo,Li Yuan,Zhai Hezheng,et al.Effects of SKP2 on the bystander effect induced by irradiated esophageal cancer cells[J].Chinese Journal of Radiological Medicine and Protection,2014,34(10):739-742 |
| Effects of SKP2 on the bystander effect induced by irradiated esophageal cancer cells |
| Received:June 27, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.10.005 |
| KeyWords:Esophageal cancer cells SKP2 Radiation-induced bystander effect |
| FundProject:国家自然科学基金（81272511）；天津市自然科学基金重点项目（14JCZCJC13700） |
| Author Name | Affiliation | E-mail | | Zhang Zhubo | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China | | | Li Yuan | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China | | | Zhai Hezheng | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China | | | Ruan Shuzhou | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China | | | Wang Xiaochun | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China | wxc3188@126.com |
|
| Hits: 3834 |
| Download times: 2556 |
| Abstract:: |
| Objective: To investigate the effect of SKP2 expression on radiation induced bystander effect (RIBE) of esophageal cancer cells. Methods: The esophageal cancer cell lines with different SKP2 levels were applied for the study and the SKP2 expression was identified by Western blot. Micronuclei (MN) assay and DNA foci assay were used to evaluate the effect of SKP2 on RIBE. The cells were transfected with SKP2 gene or SKP2 siRNA to further verify the effect of SKP2 on RIBE. Results: MN assay showed that the bystander effect induced by the cells with a high level of SKP2 was lower than that induced by the cells with a lower level of SKP2 (t=8.06, P<0.01). These results were further confirmed by the gene transfection experiments. When the expression of SKP2 was increased, RIBE was decreased (t=11.12, 10.16, P<0.01). Contrarily, when the expression of SKP2 was reduced, RIBE was increased (t=8.39, 8.83, P<0.01). γ-H2AX foci formation assay disclosed that when SKP2 expression in the irradiated cells increased, the repair ability of DNA damage in the bystander cells was higher than the control (t=6.85, 7.10, P<0.01). With the expression of SKP2 decreased, the repair ability of DNA damage was lower than the control (t=7.66, 8.47, P<0.01). Conclusions: Over-expression of SKP2 inhibits RIBE of esophageal cancer cells, at least partly through regulating DNA damage repair ability. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
