| Chang Pengyu,Cui Shuang,Luo Jinghua,et al.Therapeutic effect of adipose-derived mesenchymal stem cells on radiation enteritis[J].Chinese Journal of Radiological Medicine and Protection,2014,34(10):729-734 |
| Therapeutic effect of adipose-derived mesenchymal stem cells on radiation enteritis |
| Received:March 31, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.10.003 |
| KeyWords:Adipose-derived mesenchymal stem cell Radiation enteritis Cell-based therapy |
| FundProject:国家自然科学基金（81372929） |
| Author Name | Affiliation | E-mail | | Chang Pengyu | Department of Radiation Oncology, First Bethune Hospital of Jilin University, Changchun 130021, China | | | Cui Shuang | Department of Radiation Oncology, First Bethune Hospital of Jilin University, Changchun 130021, China | | | Luo Jinghua | Department of Radiation Oncology, First Bethune Hospital of Jilin University, Changchun 130021, China | | | Qu Chao | Department of Radiation Oncology, First Bethune Hospital of Jilin University, Changchun 130021, China | | | Jiang Xin | Department of Radiation Oncology, First Bethune Hospital of Jilin University, Changchun 130021, China | | | Qu Yaqin | Department of Radiation Oncology, First Bethune Hospital of Jilin University, Changchun 130021, China | | | Dong Lihua | Department of Radiation Oncology, First Bethune Hospital of Jilin University, Changchun 130021, China | drlhdong@163.com |
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| Abstract:: |
| Objective: To evaluate the therapeutic effect of adipose-derived mesenchymal stem cells on radiation enteritis. Methods: A total of 52 male Sprague-Dawley rats were used in the present study. Herein, 46 rats were randomly selected and irradiated with a dose of 15 Gy at their abdomens. Two hours post-irradiation, 23 rats were randomly selected and infused intraperitoneally with adipose-derived mesenchymal stem cells in passage 6 from young-female donor. The other 23 rats were intraperitoneally infused with PBS. The rest 6 rats were set as normal control. During the first 10 days post-irradiation, peripheral blood-samples from irradiated rats were harvested for testing the levels of IL-10 in serum using ELISA assay. Additionally, after isolating the thymic cells and peripheral blood mononuclear cells, the percentages of CD4/CD25/Foxp(3)-positive regulatory T cells in thymus and peripheral blood were tested by flow-cytometry. Finally, infiltration of inflammatory cells and deposition of collagens within irradiated small intestine were analyzed by H&E staining and Masson Trichrome staining, respectively. Based on the MPO-immunohistochemistry staining, the type of infiltrated cells was identified. The Kaplan-Meier method was used for analyzing the survival rate of irradiated rats. Results: During a period of 30 days post-irradiation, the irradiated rats receiving adipose-derived mesenchymal stem cells survived longer than those receiving PBS (t=4.53, P<0.05). Compared to the irradiated rats with PBS-treatment, adipose-derived mesenchymal stem cells could elevate the level of IL-10 in serum (7 d: t=13.93, P<0.05) and increase the percentages of CD4/CD25/Foxp(3)-positive regulatory T cells in both peripheral blood (3.5 d: t=7.72, 7 d: t=11.11, 10 d: t=6.99, P<0.05) and thymus (7 d: t=16.17, 10 d: t=12.12, P<0.05). Moreover, infiltration of inflammatory cells and deposition of collagens within irradiated small intestine were mitigated by adipose-derived mesenchymal stem cells. Conclusions: Adipose-derived mesenchymal stem cells were capable of curing radiation enteritis. |
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