| ,et al.X-ray irradiation modulates the expressions of free ubiquitin in the serum, small intestine, and heart of C57 BL/6N mice[J].Chinese Journal of Radiological Medicine and Protection,2014,34(9):668-671 |
| X-ray irradiation modulates the expressions of free ubiquitin in the serum, small intestine, and heart of C57 BL/6N mice |
| Received:December 20, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.09.006 |
| KeyWords:Ionizing radiation Free ubiquitin |
| FundProject:国家自然科学基金（81172597，31300694）；江苏省高校重大科研项目（11KJA310001） |
| Author Name | Affiliation | E-mail | | 徐静 | School of Radiation Medicine and Protection, Medical Collede of Soochow University, Suzhou 215123, China | | | 谷庆 | School of Radiation Medicine and Protection, Medical Collede of Soochow University, Suzhou 215123, China | | | 何燕 | School of Radiation Medicine and Protection, Medical Collede of Soochow University, Suzhou 215123, China | | | 杜傲男 | School of Radiation Medicine and Protection, Medical Collede of Soochow University, Suzhou 215123, China | | | 曹晗 | School of Radiation Medicine and Protection, Medical Collede of Soochow University, Suzhou 215123, China | | | 徐加英 | School of Radiation Medicine and Protection, Medical Collede of Soochow University, Suzhou 215123, China | | | 朱巍 | School of Radiation Medicine and Protection, Medical Collede of Soochow University, Suzhou 215123, China | | | 李明 | School of Radiation Medicine and Protection, Medical Collede of Soochow University, Suzhou 215123, China | | | 张舒羽 | School of Radiation Medicine and Protection, Medical Collede of Soochow University, Suzhou 215123, China | | | 杨红英 | School of Radiation Medicine and Protection, Medical Collede of Soochow University, Suzhou 215123, China | | | 曹建平 | School of Radiation Medicine and Protection, Medical Collede of Soochow University, Suzhou 215123, China | jpcao@suda.edu.cn |
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| Abstract:: |
| Objective To investigate the effect of X-ray irradiation on the expression of free ubiquitin in the serum, small intestine, and heart tissues of C57 BL/6N mice.Methods The amount of free ubiquitin protein in the serum and tissue homogenates was analyzed quantitatively with ELISA and Western Blotting assay. The mRNA expressions of free ubiquitin in the tissues were detected by RT-PCR.Results At 24 or 48 h after radiation, the free ubiquitin level in the serum and small intestine tissue increased asymptotically with increasing of radiation dose (F=183.1, 435.3, P<0.01). After 5 and 10 Gy X-ray irradiation, the concentration of serum free ubiquitin increased asymptotically with the extended response time (F=131.4, 442.9, P<0.01). Compared with the normal control group, at 24 h after 10 Gy X-ray irradiation, the expressions of free ubiquitin protein and mRNA in small intestine tissue were significantly up-regulated (t=-18.7, -10.1, P<0.01). However, there was no difference in the expressions of free ubiquitin protein and mRNA in the heart tissues between irradiated and nonirradiated groups (t=-2.0, 3.1, P>0.05). Conclusions Because of the high expressions of free ubiquitin protein in the radiosensitive mice tissues, X-ray radiation could increase the concentration of free ubiquitin in serum. The changes of free ubiquitin may be related to cellular radiosensitivity and tissue injury. |
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