| .Adipose-derived mesenchymal stem cell therapy for radiation-induced vascular injury in small intestine of rat[J].Chinese Journal of Radiological Medicine and Protection,2014,34(9):652-657 |
| Adipose-derived mesenchymal stem cell therapy for radiation-induced vascular injury in small intestine of rat |
| Received:March 27, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.09.003 |
| KeyWords:Adipose-derived mesenchymal stem cells Radiation-induced intestinal injury Blood vessel Repairation |
| FundProject:国家自然科学基金（81372929） |
| Author Name | Affiliation | E-mail | | 常鹏宇 | Department of Radiation Oncology, First Bethune Hospital of Jilin University, Changchun 130021, China | | | 崔爽 | Department of Radiation Oncology, First Bethune Hospital of Jilin University, Changchun 130021, China | | | 姜新 | Department of Radiation Oncology, First Bethune Hospital of Jilin University, Changchun 130021, China | | | 曲超 | Department of Radiation Oncology, First Bethune Hospital of Jilin University, Changchun 130021, China | | | 蒋鑫萍 | Department of Radiation Oncology, First Bethune Hospital of Jilin University, Changchun 130021, China | | | 罗景华 | Department of Radiation Oncology, First Bethune Hospital of Jilin University, Changchun 130021, China | | | 曲雅勤 | Department of Radiation Oncology, First Bethune Hospital of Jilin University, Changchun 130021, China | quyaqin52@163.com | | 董丽华 | Department of Radiation Oncology, First Bethune Hospital of Jilin University, Changchun 130021, China | |
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| Abstract:: |
| Objective To assess the therapeutic effect of human adipose-derived mesenchymal stem cells on radiation-induced vascular injury in the small intestine of rat.Methods A total of 34 male Sprague-Dawley rats were enrolled in this study. To establish a model of radiation-induced intestinal injury, each rat was irradiated with 15 Gy in whole abdomen. 17 rats were randomly selected and infused intraperitoneally with passage 6 (P6) Ad-MSCs, and the other 17 rats that received PBS were set as control. 10 days post-irradiation, the number of CD31+ endothelial cells in the small intestine villus was measured by flow-cytometry, the expressions of CD31, CD105 and isolectin-B4 in the nave endothelial cells with detected by IHC-staining, and the vascular integrity was evaluated by measuring VE-Cadherin. The origination of nave endothelial cells within injured intestine was also analyzed. In addition, total mRNA were extracted from irradiated small intestine to assay the expressions of VEGF, bFGF, Flk-1 and SDF-1 using quantitative Real-time PCR.Results Compared to the control, the amount of CD31-postive endothelial cells within irradiated intestine was significantly increased after Ad-MSCs infusion (t=12.15, P<0.05). The microvascular density in the injured sites was also significantly increased by the infusion of Ad-MSCs (20 d: t=10.33, P<0.05;30 d: t=32.85, P<0.05). Moreover, the expressions of VEGF, bFGF, Flk-1 and SDF-1 were significantly up-regulated after delivery of Ad-MSCs (VEGF:t=10.34,bFGF:t=11.25,Flk-1:t=6.73, SDF-1:t=6.73, all P<0.05), which was beneficial in maintaining the integrity of intra-villus blood-vessels as well as promoting neovascularization in the injured sites.Conclusion Ad-MSCs had potentials in healing radiation-induced vascular injury in rat small intestine. |
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