| Xue Mengchen,Cai Xuwei,Liu Qi,et al.Experimental studies of the correlation between vitronectin and collagen expressions post-irradiation[J].Chinese Journal of Radiological Medicine and Protection,2014,34(8):573-577 |
| Experimental studies of the correlation between vitronectin and collagen expressions post-irradiation |
| Received:October 28, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.08.004 |
| KeyWords:Vitronectin Collagen Biological Markers Radiation Fibroblast |
| FundProject:国家自然科学基金（81000993）；2010上海市卫生局青年科研项目（2010Y016） |
| Author Name | Affiliation | E-mail | | Xue Mengchen | Department of Radiation Oncology, Fudan University Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China | | | Cai Xuwei | Department of Radiation Oncology, Fudan University Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China | birdhome2000@163.com | | Liu Qi | Department of Radiation Oncology, Fudan University Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China | | | Zhang Qin | Department of Radiation Oncology, Fudan University Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China | | | Xie Congying | 温州医科大学附属第一医院放化疗科 | | | Fu Xiaolong | Department of Radiation Oncology, Fudan University Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China | |
|
| Hits: 3775 |
| Download times: 2298 |
| Abstract:: |
| Objective To analyze radiation induced alterations of vitronectin and collagen expressions in fibroblasts at different times post-irradiation, so as to evaluate the potential to apply vitronectin as a biomarker of radiation-induced lung fibrosis. Methods The human fibroblast cells WI-38 and IMR-90 were irradiated with 137Cs γ-rays at doses of 0 (control), 4, 6, 8, 10 and 12 Gy, respectively. The cells and its supernatant were collected at 6, 12, 24, 36, 48 and 60 h post-irradiation. The expressions of vitronectin and collagen Ⅰ and Ⅲ were analyzed by Western blot, PCR and ELISA. Results After irradiation, the expressions of vitronectin and collagen Ⅰ and Ⅲ were positively correlated (r=0.40-0.79, P<0.05) and were all significantly higher than that in control group (t=3.04-25.45,P<0.05) and reached the highest expression levels at 48 h after 8-10 Gy of irradiation (t=2.92-18.86,P<0.05). Analyses of Real-time PCR and ELISA assay showed that expressions of vitronectin mRNA and its protein level in the cell lysis were significantly increased by radiation (F=27.09-42.62, P<0.05). Conclusions The expressions of vitronectin in cellular supernatant and its mRNA may be a potential biomarker of radiation-induced fibrosis, and 48 h after 8 Gy irradiation may be an optimum condition of measurement. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
