| Lin Kaili,Xu Chang,Du Liqing,Wang Yan,Li Deguan,Liu Jianxiang,Su Xu,Fan Saijun,Fan Feiyue,Liu Qiang.The effect of Sirt3 mediated SOD2 in radiation responses[J].Chinese Journal of Radiological Medicine and Protection,2014,34(8):569-572 |
| The effect of Sirt3 mediated SOD2 in radiation responses |
| Received:January 02, 2014 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.08.003 |
| KeyWords:Silent information regulator 3 Superoxide dismutase 2 Reactive oxygen species 293 T cells Ionizing radiation |
| FundProject:卫生部卫生行业科研专项（201002009）；国家自然科学基金（31240052）；天津市自然科学基金（13JCYBJC23500，13JCQNJC11600）；协和青年基金资助和中央高校基本科研业务费专项资金（2012G01） |
| Author Name | Affiliation | E-mail | | Lin Kaili | Department of Radiation Hazard Evaluation, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical College, Tianjin Key Lab of Molecular Nuclear Medicine, Tianjin 300192, China | | | Xu Chang | Department of Radiation Hazard Evaluation, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical College, Tianjin Key Lab of Molecular Nuclear Medicine, Tianjin 300192, China | | | Du Liqing | Department of Radiation Hazard Evaluation, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical College, Tianjin Key Lab of Molecular Nuclear Medicine, Tianjin 300192, China | | | Wang Yan | Department of Radiation Hazard Evaluation, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical College, Tianjin Key Lab of Molecular Nuclear Medicine, Tianjin 300192, China | | | Li Deguan | Department of Radiation Hazard Evaluation, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical College, Tianjin Key Lab of Molecular Nuclear Medicine, Tianjin 300192, China | | | Liu Jianxiang | 中国疾病预防控制中心辐射防护与核安全医学所 辐射防护与核应急中国疾病预防控制中心重点实验室 | | | Su Xu | 中国疾病预防控制中心辐射防护与核安全医学所 辐射防护与核应急中国疾病预防控制中心重点实验室 | | | Fan Saijun | Department of Radiation Hazard Evaluation, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical College, Tianjin Key Lab of Molecular Nuclear Medicine, Tianjin 300192, China | | | Fan Feiyue | Department of Radiation Hazard Evaluation, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical College, Tianjin Key Lab of Molecular Nuclear Medicine, Tianjin 300192, China | | | Liu Qiang | Department of Radiation Hazard Evaluation, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical College, Tianjin Key Lab of Molecular Nuclear Medicine, Tianjin 300192, China | liuqiang@irm-cams.ac.cn |
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| Abstract:: |
| Objective To study the effects of the silent information regulator 3 (sirt3) and its relative anti-oxidation factors on ionizing radiation (IR)-induced damage in 293T cells. Methods 293T cells irradiated with 2, 4, 8, 16 Gy of γ -rays, after 6, 12, 24 and 48 h of exposure, the expressions of sirt3 mRNA and SOD2 protein were examined by Real-Time PCR and immunoblotting assay, respectively. sirt3 siRNA was applied to knock down sirt3 in the cells. SOD2 activities were measured by a WST kit and the cellular ROS level was detected by a flow cytometry. Results The mRNA levels of sirt3 and SOD2 in each group campared with the 0 Gy group were reached the maximum at 12 h(t=6.75, 13.59, 6.59, 10.13,P<0.05)and 24 h(t=6.80, 8.73, 11.09,P<0.05), and protein levels also increased. It was found that sirt3 was a upstream regulatory factor of SOD2. The activity of SOD2 were much higher at 24 h(t=46.04, 23.19, 26.28, 14.70,P<0.05)than that in the 0 Gy group as well as the level of ROS. Conclusion Sirt3 may strengthen antioxidant protection system through regulation of the SOD2 expression and activity, and possibly plays an important role in IR-induced damage response and clinic treatment. |
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