| Dang Xuhong,Zuo Yahui,Yuan Yayi,et al.Effects of HAVCR2 gene silence on apoptosis and cell cycle in the radiation-caused genomic instable liver cells[J].Chinese Journal of Radiological Medicine and Protection,2014,34(7):502-506 |
| Effects of HAVCR2 gene silence on apoptosis and cell cycle in the radiation-caused genomic instable liver cells |
| Received:August 02, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.07.006 |
| KeyWords:HAVCR2 gene Genomic instability RNA interference Cell cycle Apoptosis |
| FundProject: |
| Author Name | Affiliation | E-mail | | Dang Xuhong | China Institute for Radiation Protection, Taiyuan 030006, China | | | Zuo Yahui | China Institute for Radiation Protection, Taiyuan 030006, China | yahuiz@163.com | | Yuan Yayi | China Institute for Radiation Protection, Taiyuan 030006, China | | | Yang Biao | China Institute for Radiation Protection, Taiyuan 030006, China | | | Wang Chao | China Institute for Radiation Protection, Taiyuan 030006, China | | | Liu Jiangong | China Institute for Radiation Protection, Taiyuan 030006, China | |
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| Abstract:: |
| Objective To explore the effects of HAVCR2 siRNA on apoptosis and cell cycle in the radiation-caused genomic instable liver cells.Methods RNAi was used to inhibit HAVCR2 gene transcription. Cellular apoptosis and cell cycle distribution were detected by flow cytometry (FCM). Expression of p53 gene was assayed by real time fluorescence quantitative PCR. Results HAVCR2 gene was effectively inhibited by RNAi(t=19.21,P<0.05). After siRNA transferring, cell apoptosis(t=3.65,P <0.05)and p53 gene expression(t=4.82,P<0.05)were decreased, and G2-phase arrest was induced(t=-3.41,P<0.05). Conclusion HAVCR2 siRNA can decrease the generation of apoptosis in the genomic instable liver cells and blocks the cells at G2 phase. |
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