| Wang Weifeng,Yuan Feng,Lin Zhiren,et al.Combination effect of radiation and p53 gene regulated by a radiation-enhanced promoter on tumor cells[J].Chinese Journal of Radiological Medicine and Protection,2014,34(7):493-496 |
| Combination effect of radiation and p53 gene regulated by a radiation-enhanced promoter on tumor cells |
| Received:July 11, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.07.004 |
| KeyWords:Irradiation Radiation enhanced promoter p53 gene |
| FundProject:海南省自然科学基金（310149） |
| Author Name | Affiliation | E-mail | | Wang Weifeng | Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China | | | Yuan Feng | Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China | | | Lin Zhiren | Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China | | | Wang Guoping | Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China | | | Yang Huagao | Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China | | | Chen Longhua | Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China | chenlhsumu@126.com |
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| Abstract:: |
| Objective To study the specific killing effect in human carcinoma cells after combination treatment of radiation and p53 gene regulated by a radiation-enhanced promoter.Methods A plasmid pE6(TATA)-p53 was constructed. After irradiation, the expression of P53 was detected with Western blot assay, apoptosis was detected by Annexin V-FITC, and cell survival was detected by clonogenic assay then the sensitivity enhancement ratio (SER) was analyzed for HeLa and A549 cells. Results The expression of P53 were increased in the irradiated cells and 6 Gy irradiation triggered the strongest activity. After p53 transfection, radiation-induced apoptosis was obviously enhanced in comparison with the control group without gene transfection(F=11.018,10.736, P<0.05). The SER of p53-promoter was 2.36 for A549 cells and 2.56 for Hela cells. Conclusions The p53-plasmid promoter could induce apoptosis and enhance the radiosensitivity of tumor cells, which may provide a novel therapeutic strategy for cancer treatment. |
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