| Chen Jing,Ma Lin,Qu Baolin,et al.Nimotuzumab and cetuximab enhanced radiation effects on human esophageal carcinoma cells[J].Chinese Journal of Radiological Medicine and Protection,2014,34(7):489-492 |
| Nimotuzumab and cetuximab enhanced radiation effects on human esophageal carcinoma cells |
| Received:August 06, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.07.003 |
| KeyWords:Epidermal growth factor receptor Nimotuzumab Cetuximab Esophageal carcinoma Irradiation |
| FundProject: |
| Author Name | Affiliation | E-mail | | Chen Jing | PLA General Hospital Cancer Center and Department of Radiotherapy, PLA Postgraduate School of Medicine, Beijing 100853, China | | | Ma Lin | PLA General Hospital Cancer Center and Department of Radiotherapy, PLA Postgraduate School of Medicine, Beijing 100853, China | | | Qu Baolin | PLA General Hospital Cancer Center and Department of Radiotherapy, PLA Postgraduate School of Medicine, Beijing 100853, China | | | Li Jianxiong | PLA General Hospital Cancer Center and Department of Radiotherapy, PLA Postgraduate School of Medicine, Beijing 100853, China | | | Lan Yuling | PLA General Hospital Cancer Center and Department of Radiotherapy, PLA Postgraduate School of Medicine, Beijing 100853, China | | | Yang Yongqiang | PLA General Hospital Cancer Center and Department of Radiotherapy, PLA Postgraduate School of Medicine, Beijing 100853, China | | | Meng Lingling | PLA General Hospital Cancer Center and Department of Radiotherapy, PLA Postgraduate School of Medicine, Beijing 100853, China | | | Feng Linchun | PLA General Hospital Cancer Center and Department of Radiotherapy, PLA Postgraduate School of Medicine, Beijing 100853, China | 301flc@163.com |
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| Abstract:: |
| Objective To observe the combination effects of radiation and nimotuzumab (h-R3) or cetuximab (C225) on human esophageal squamous carcinoma cell line TE-13.Methods TE-13 cells were divided into 6 groups, including no treatment control, h-R3, C225, irradiation, h-R3 combined with irradiation, and C225 combined with irradiation. For each group, cell proliferation was evaluated by MTS assay, cell radiosensitivity was determined by clonogenic assay and the survival curve was fitted with the multi-targets single-hit model, and cell cycle distribution and apoptosis were analyzed with flow cytometry assay. Results Compared with the irradiation group, after the combination treatment of mAbs and irradiation, the cell proliferation, the cell proliferations were obviously decreased (F=325.59,P<0.05), SF2, D0, Dq, and N values were obviously decreased, the cell apoptosis rates were significantly increased (F=120.59,P<0.05), and the percentages of cells in G0/G1 and G2/M phases were increased. The cell apoptosis rate of the C225 combined with irradiation group was higher than that of the h-R3 combined with irradiation group (F=120.59,P<0.05). The radiosensitization enhancement ratio (SER) of C225 was 1.83, higher than that of h-R3. Conclusions Both h-R3 and C225 can significantly enhance radiation damage of TE-13 cells and C225 is more effective than h-R3. |
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